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Auto2D® Automated 2-D Gel Electrophoresis Device Protocol
The Auto2D® Automated 2-D Gel Electrophoresis Device video and detailed protocol provide reliable methods to prepare your samples and operate the Auto2D® Device.
Brilliant Blue G – Colloidal Concentrate Protocol
Brilliant Blue G-Colloidal Concentrate stains proteins in IEF, PAGE, and SDS-PAGE gels after electrophoresis, enhancing sensitivity with protein fixation.
Cleaning Up Samples using 2-D Clean-Up Kit
2-D Clean-Up Kit from Cytiva is designed to prepare samples that would otherwise produce poor 2-D results.
Applying Equilibrated Immobiline® DryStrip Gels
This page describes how to apply equilibrated Immobiline® DryStrip gels on the ExcelGel SDS gels.
Hand-casting gels for PAGE and SDS-PAGE using mPAGE® TurboMix Bis-Tris Gel Casting Kits
Simplify your SDS-PAGE gel preparation with our pre-mixed solutions and protocols to achieve precise protein separation.
Inserting Gels into Ettan™ DALT Electrophoresis Units
This protocol shows how to insert gels into Ettan™ DALT electrophoresis units.
Paper-Bridge Loading
This page shows how to apply higher sample volumes and protein amounts with paper bridges in isoelectric focusing with Cytiva products
Preparing for IEF
This protocol shows how to prepare the Immobiline® DryStrip Kit and electrodes strips.
ReadyBlue Protein Gel Staining Protocols
ReadyBlue™ Protein Gel Stain offers rapid Coomassie® staining for polyacrylamide gels with a simplified protocol.
Rehydration Solutions
This page describes typical rehydration solutions for use in gel electrophoresis with Cytiva products.
How to Run an mPAGE™ Protein Gel Using a Bio-Rad Electrophoresis System
Instructions for setting up an mPAGE SDS-PAGE precast protein gel for gel electrophoresis using a Bio-Rad Mini-PROTEAN® electrophoresis system.
How to Run an mPAGE™ Protein Gel Using an Invitrogen XCell Surelock® Mini-Cell Electrophoresis System
Instructions for setting up an mPAGE™ SDS-PAGE precast protein gel for gel electrophoresis using an Invitrogen XCell Surelock® mini-cell.
Introduction to SDS-PAGE - Separation of Proteins Based on Size
Introduction to PAGE. Learn about SDS-PAGE background and protocol for the separation of proteins based on size in a poly-acrylamide gel.
Northern & Southern Blot Protocols
Learn Northern and Southern blotting basics, with protocols and applications for macromolecule transfer to membrane supports.
TAE and TBE Running Buffers Recipe & Video
TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.
Troubleshooting first-dimension IEF: Ettan™ IPGphor™ 3 Isoelectric Focusing System
Troubleshooting first-dimension isoelectric focusing with Ettan IPGphor 3 Isoelectric Focusing System from Cytiva.
Two-Dimensional Separation of Protein Samples
This page describes two-dimensional separation of protein samples with Cytiva products.
Milli-Q® IX Pure Lab Water System
Discover Milli-Q® IX systems for high-purity water. Ideal for general lab & sensitive techniques. Reliable Efficient Innovative. A Greener Alternative.
Gel Electrophoresis
Protein gel electrophoresis methods are used for protein separation in preparation for downstream applications like Western blotting and mass spectrometry.
Precipitation Procedures
This page describes protein precipitation as an optional step in sample preparation for 2-D electrophoresis with Cytiva products.
Proteomic analysis of membrane proteins
This page shows how to perform a proteomic analysis of membrane proteins to identify and quantitate all proteins expressed in a cell or a tissue with products from Cytiva.
Performing a Purity and Homogeneity Check
This page shows how to perform a purification and homogeneity check of membrane proteins with products from Cytiva.
Fluorescent Multiplex Detection using Antibody Atto Dye Conjugates
Immunoblotting (Western blot transfer) is a common technique in modern proteomics research.
Protein Visualization
Prior to probing a membrane using precious antibodies, it is helpful to visualize the transferred proteins on the blot to ensure complete transfer and even loading. This page describes possible causes and potential remedies for challenges encountered during protein visualization.
Winning Westerns Technique Talk: Perfecting Western Blotting
In this webinar, Alli Lynch, Ph.D. reviews the basics of Western blotting, identifies common troubleshooting issues, and provides tips for Western blotting success.
Applying Equilibrated Immobiline® DryStrip Gels to SDS Gels
This page shows how to apply equilibrated Immobiline DryStrip gels
Auto2D® 2-D Gel Electrophoresis Device Frequently Asked Questions
Auto2D® 2-D Gel Electrophoresis Device frequently asked questions for 2-D PAGE and additional 2DE protein separation workflow needs.
Auto2D® Gel Electrophoresis Device
Auto2D® automates difficult 2D electrophoresis methods for protein localization with high reproducibility in under two hours.
Bis Tris Polyacrylamide Gel Electrophoresis Technology
Bis-Tris SDS-PAGE gel casting kits with mPAGE® TurboMix technology for rapid, reliable gel casting and protein separation.
Components of Rehydration Solution
This page describes components of rehydration solutions for use in 2-D Electrophoresis with Cytiva products.
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