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Molecular Biology & Genomics Learning Hub

Introduction

From routine cloning, rapid nucleic acid purification, reliable PCR amplification, and novel CRISPR gene editing tools, get the time and resource-saving benefits of our molecular tools that are ready when you are. Our highly proven molecular reagents provide a range of “grab-and-go” tools so that you can select from a variety of gold-standard offerings and solutions that scale from bench-top R&D to production-level needs. Explore technical resources for cloning, expression, nucleic acid purification, PCR, gene editing, and NGS applications. 

Section Overview

Featured Articles

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Blue-white color selection of recombinant bacteria using X-gal.
Blue-White Screening & Protocols for Colony Selection

Learn about and find protocols for the blue white screen technique used in molecular biology research to identify recombinant bacterial clones for further analysis.

Lab worker looking at a bottle of fluid on a shelf.
Custom DNA Oligos: Standard DNA Synthesis

High-quality custom DNA oligos made to meet your specifications (scale, purification, length, dry/solution, modifications, tubes/plates).

Two lab workers looking at a computer screen.
PCR/qPCR Data Analysis

After a traditional PCR has been completed, the PCR/qPCR data analysis is conducted by resolution through an agarose gel or, more recently, through a capillary.

Purple glove and pipette
Quantitative PCR Basics

Quantitative PCR detection builds on basic PCR techniques and allows researchers to estimate the quantity of starting material in a sample with a much wider dynamic range.

Pipette and tray.
Polymerase Chain Reaction

Polymerase chain reaction is one of the most widely used techniques in molecular biology with 3 main steps: denature, anneal, and extend.

Lab worker pipetting at a work station.
T7 Promoter System

The T7 promoter is commonly used to regulate gene expression of recombinant proteins, which can be subsequently used for a variety of downstream research applications.

Genomic Techniques & Technologies

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Sample tubes being loaded into a PCR thermocycler
PCR Applications

Polymerase chain reaction (PCR) is a technique for amplifying nucleic acid molecules and is commonly used in many applications, including RT-PCR, hot start PCR, end point PCR and more.

Colorful strands of DNA.
DNA & RNA Purification

Overview of common techniques and downstream applications for extraction and purification of genomic DNA, plasmid DNA, and total RNA from cells, blood, viruses, and more.

Lab worker at his station.
Epigenetics

Epigenetics tools and techniques for researching the complexities of epigenetic mechanisms including DNA methylation, histone modification, and RNA regulation.

Purple glove and pipette
qPCR

Quantitative PCR (qPCR) or quantitative Real-Time PCR applications, utilizing fluorescent report molecules, suitable for your microRNA, genotyping, and gene expression analyses.

Lab technician working with lab equipment.
Nucleic Acid Gel Electrophoresis

Learn the basics of DNA and RNA gel electrophoresis, and how these techniques can be used to separate and purify DNA and RNA molecules based on size and charge.

Lab worker pipetting at a work station.
Gene Expression & Silencing

CRISPR, miRNA, siRNA, and shRNA technologies for gene expression and gene silencing applications.

Resources Library

Recommended Readings

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Sample tubes being loaded into a PCR thermocycler
RT-qPCR – Quantitative Reverse Transcription PCR

RT-qPCR detects specific targets with applications in gene expression and pathogen detection.

Lab worker talking to a coworker.
Sanger Sequencing Steps & Methods

Learn about Sanger Sequencing steps or the chain termination method and how DNA sequencing works and how to read Sanger Sequencing results accurately for your research.

Amino acid codon wheel.
Amino Acid Codon Wheel

Amino Acid Codon Wheel for fast RNA translation. Find which amino acid is translated from your RNA sequence quickly and easily.

Sample tubes being loaded into a PCR thermocycler
Standard PCR

Learn standard PCR protocol steps and review reagent lists or cycling parameters. This method for routine PCR amplification of DNA uses standard Taq DNA polymerase.

Lab workers talking.
RT-PCR Troubleshooting

PCR troubleshooting guide for reliable data in molecular assays.

Universal SYBR Green qPCR graphic.
Universal SYBR Green qPCR

Our SYBR Green qPCR Protocol is a method designed to detect accurate quantification of gene expression and RT-PCR reactions.

Webinars

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Section Overview