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SCM105 3dGRO™ L-WRN Conditioned Media Supplement

L-WRN conditioned media supplement containing the 3 growth factors Wnt3a (W), R-spondin-3 (R), and Noggin (N) for organoid cultures.

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SCM105
50 mL  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCM105
      Description3dGRO™ L-WRN Conditioned Media Supplement
      Alternate Names
      • WRN Conditioned Media
      • L-WRN CM
      Background InformationThe 3dGRO™ L-WRN Conditioned Media Supplement is a formulation based on DMEM/F-12 PLUS medium containing the 3 growth factors Wnt3a (W), R-spondin-3 (R), and Noggin (N). These factors act upon the Wnt signaling pathway, which plays a critical role in sustaining the proliferation of LGR5+ crypt stem cells within organoids. Wnt activation is thus essential for in vitro growth of human gut organoids1. L-WRN conditioned medium is widely used as a component of the culture medium for supporting the growth of human intestinal organoids2 including the 3dGRO™ gastrointestinal organoid biobank (SCC310-SCC367). Applications for L-WRN conditioned medium include but are not limited to the expansion of human intestinal gut organoids and other epithelial organoid cell types.

      Note: Product is supplied at a 2X concentration (see datasheet for a complete organoid media recipe).

      Source
      The 3dGRO™ L-WRN Conditioned Media Supplement is derived from the Mus musculus L-M(TK-) cell line secreting mouse Wnt3a, R-spondin-3 and Noggin. L-WRN Conditioned Medium may contain trace amounts of mouse DNA.
      References
      Product Information
      Applications
      ApplicationL-WRN conditioned media supplement containing the 3 growth factors Wnt3a (W), R-spondin-3 (R), and Noggin (N) for organoid cultures.
      Key Applications
      • Cell Culture
      • Stem Cell Culture
      Biological Information
      Gene Symbol
      • WNT3A
      • RSPO3
      • NOG
      Media FormLiquid
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality Assurance• Appearance (color): clear, red liquid
      • Endotoxin: <2 EU/mL
      • Sterility Tested: No Growth/Pass
      • Media are tested for function in supporting growth of human intestinal organoids
      • Media are tested for levels of growth factors by ELISA
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsThe 3dGRO™ L-WRN Conditioned Media Supplement should be stored at -20°C. When ready to use, thaw the L-WRN Conditioned Medium overnight at 2-8°C in the dark. Thawed medium is good for two weeks when stored at 2-8°C. If media will not be used within two weeks, aliquot unused media and store at -20°C. After thawing refrozen aliquots, do not re-freeze media.
      Packaging Information
      Material Size50 mL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCM105 04061842403455