100128 Sigma-AldrichA77 1726 - CAS 108605-62-5 - Calbiochem

Leflunomide metabolite that blocks TNF-dependent NF-κB activation and gene expression.

A77 1726 - CAS 108605-62-5 - Calbiochem MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

SDS
CoA
References
Data Sheet

Synonyms: N-(4-Trifluoromethylphenyl)-2-cyano-3-hydroxycrotoamide

Primary Target: Dihydroorotate dehydrogenase (DHO-DH)

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Overview

Replacement Information

Key Spec Table

CAS #	Empirical Formula
108605-62-5	C₁₂H₉F₃N₂O₂

Pricing & Availability

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Description
Overview	Leflunomide metabolite that blocks TNF-dependent NF-κB activation and gene expression. Inhibits the activity of dihydroorotate dehydrogenase (DHO-DH; IC₅₀ = 220 nM). Reported to reduce IFN-γ-induced expression of iNOS in L929 fibroblasts. Inhibits IL-1β-induced activation of COX-2 and PGE₂ synthesis (IC₅₀= 130 nM) in A549 cells. A cell-permeable, reversible, and substrate competitive inhibitor that inhibits the IL-2-induced tyrosine phosphorylation of JAK1 and JAK3.
Catalogue Number	100128
Brand Family	Calbiochem®
Synonyms	N-(4-Trifluoromethylphenyl)-2-cyano-3-hydroxycrotoamide

References
References	Jankovic, V., et al. 2000. Cell Immunol. 199, 73. Hamilton, L.C., et al. 1999. Br. J. Pharmacol. 127, 1589. Manna, S.K., and Aggarwal, B.B. 1999. J. Immunol. 162, 2095. Elder, R.T., et al. 1997. J. Immunol. 159, 22. Xu, X., et al. 1996. Biochem. Pharmacol. 52, 527. Xu, X., et al. 1995. J. Biol. Chem. 270, 12398. Mattar, T., et al. 1993. FEBS Lett. 334, 161.

Product Information
CAS number	108605-62-5
ATP Competitive	N
Form	White solid
Hill Formula	C₁₂H₉F₃N₂O₂
Chemical formula	C₁₂H₉F₃N₂O₂
Reversible	Y
Structure formula Image
Quality Level	MQ100

Applications

Biological Information
Primary Target	Dihydroorotate dehydrogenase (DHO-DH)
Primary Target IC<sub>50</sub>	220 nM against DHO-DH
Purity	≥97% by HPLC

Physicochemical Information
Cell permeable	Y

Dimensions

Materials Information

Toxicological Information

Safety Information according to GHS

Safety Information
R Phrase	R: 40 Limited evidence of a carcinogenic effect.
S Phrase	S: 22-36 Do not breathe dust. Wear suitable protective clothing.

Product Usage Statements

Storage and Shipping Information
Ship Code	Ambient Temperature Only
Toxicity	Carcinogenic / Teratogenic
Storage	+2°C to +8°C
Protect from Light	Protect from light
Do not freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C

Packaging Information
Packaged under inert gas	Packaged under inert gas

Transport Information

Supplemental Information

Specifications

Documentation

A77 1726 - CAS 108605-62-5 - Calbiochem SDS

Title
Safety Data Sheet (SDS)

A77 1726 - CAS 108605-62-5 - Calbiochem Certificates of Analysis

Title	Lot Number
100128	Enter Lot Number

References

Reference overview
Jankovic, V., et al. 2000. Cell Immunol. 199, 73. Hamilton, L.C., et al. 1999. Br. J. Pharmacol. 127, 1589. Manna, S.K., and Aggarwal, B.B. 1999. J. Immunol. 162, 2095. Elder, R.T., et al. 1997. J. Immunol. 159, 22. Xu, X., et al. 1996. Biochem. Pharmacol. 52, 527. Xu, X., et al. 1995. J. Biol. Chem. 270, 12398. Mattar, T., et al. 1993. FEBS Lett. 334, 161.

Data Sheet

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision	11-January-2010 RFH
Synonyms	N-(4-Trifluoromethylphenyl)-2-cyano-3-hydroxycrotoamide
Description	Leflunomide metabolite that blocks TNF-dependent NF-κB activation and gene expression. Inhibits the activity of dihydroorotate dehydrogenase (DHO-DH; IC₅₀ = 220 nM). Reported to reduce IFN-γ-induced expression of iNOS in L929 fibroblasts. Inhibits IL-1β-induced activation of COX-2 and PGE₂ synthesis (IC₅₀= 130 nM) in A549 cells.A cell-permeable, reversible and substrate competitive inhibitor that inhibits the IL-2-induced tyrosine phosphorylation of JAK1 and JAK3.
Form	White solid
Intert gas (Yes/No)	Packaged under inert gas
CAS number	108605-62-5
Chemical formula	C₁₂H₉F₃N₂O₂
Structure formula
Purity	≥97% by HPLC
Solubility	DMSO (35 mg/ml)
Storage	Protect from light +2°C to +8°C
Do Not Freeze	Ok to freeze
Special Instructions	Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C
Toxicity	Carcinogenic / Teratogenic
References	Jankovic, V., et al. 2000. Cell Immunol. 199, 73. Hamilton, L.C., et al. 1999. Br. J. Pharmacol. 127, 1589. Manna, S.K., and Aggarwal, B.B. 1999. J. Immunol. 162, 2095. Elder, R.T., et al. 1997. J. Immunol. 159, 22. Xu, X., et al. 1996. Biochem. Pharmacol. 52, 527. Xu, X., et al. 1995. J. Biol. Chem. 270, 12398. Mattar, T., et al. 1993. FEBS Lett. 334, 161.