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MAB3503-I Anti-Apaf-1 Antibody, clone 2E12

This rat monoclonal Anti-Apaf-1 Antibody, clone 2E12, Cat. No. MAB3503-I, has been tested for use in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection Apaf-1.

Anti-Apaf-1 Antibody, clone 2E12 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Apoptosis & Cancer Gene Symbol: APAF-1 UniProt Number: O14727
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MAB3503-I
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HICC, IP, EM, WBRPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMAB3503-I
      DescriptionAnti-Apaf-1 Antibody, clone 2E12
      Alternate Names
      • Apoptotic protease-activating factor 1
      Background InformationApoptotic protease-activating factor 1 (UniProt: O14727; also known as Apaf-1) is encoded by the Apaf-1 gene (Gene ID: 317) in human. Apaf-1 contains a caspase recruitment domain (CARD), an ATPase domain (NB-ARC), few short helical domains and then several copies of the WD40 repeat domain. It is highly expressed in lung and spleen, weakly in brain and kidney and is not detectable in liver. Under basal conditions it is a monomeric protein, but oligomerizes under apoptotic conditions. The monomeric form is auto-inhibited in a closed conformation through a bound ADP at the nucleotide binding site. Under apoptosis signals ADP is exchanged for ATP and its binding to cytochrome c triggers a conformation change where WD repeat region swings out, which allows the NB-ARC domain to rotate and expose the contact areas for oligomerization. Oligomeric form is a heptameric ring that binds to cytochrome c and ATP and is known as the apoptosome. Oligomeric Apaf-1 mediates the cytochrome c-dependent autocatalytic activation of pro-caspase-9 to mature caspase-9, which then activates effector caspases 3, 6, and 7.
      References
      Product Information
      FormatPurified
      PresentationPurified rat monoclonal antibody IgG2a in PBS with 0.02% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis rat monoclonal Anti-Apaf-1 Antibody, clone 2E12, Cat. No. MAB3503-I, has been tested for use in Electron Microscopy, Immunocytochemistry, Immunoprecipitation, and Western Blotting for the detection Apaf-1.
      Key Applications
      • Immunocytochemistry
      • Immunoprecipitation
      • Electron Microscopy
      • Western Blotting
      Application NotesWestern Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

      Western Blotting Analysis: A representative lot detected Apaf-1 in Western Blotting applications (Moriishi, K., et. al. (1999). Proc Natl Acad Sci USA. 96(17):9683-8).

      Immunocytochemistry Analysis: A representative lot detected Apaf-1 in Immunocytochemistry applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).

      Immunoprecipitation Analysis: A representative lot detected Apaf-1 in Immunoprecipitation applications (Moriishi, K., et. al. (1999). Proc Natl Acad Sci USA. 96(17):9683-8).

      Electron Microscopy Analysis: A representative lot detected Apaf-1 in Electron Microscopy applications (Hausmann, G., et. al. (2000). J Cell Biol. 149(3):623-34).
      Biological Information
      ImmunogenRecombinant human Apaf-1 (aa 1-464) containing the N-terminal CARD and CED-4 homologous domains.
      EpitopeN-terminus
      Clone2E12
      ConcentrationPlease refer to lot specific datasheet.
      HostRat
      SpecificityClone 2E12 detects Apaf-1 in Jurkat cells. It targets an epitope within first 97 amino acids from the N-terminal region.
      IsotypeIgG2aκ
      Species Reactivity
      • Human
      Species Reactivity NoteHuman.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • APAF-1
      Purification MethodProtein G purified
      UniProt Number
      Molecular Weight~140 kDa observed; 141.84 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in Jurkat cell lysate.

      Western Blotting Analysis: 1 µg/mL of this antibody detected Apaf-1 in 10 µg of Jurkat cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MAB3503-I 04054839198991