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MABN69 Anti-Caspr Antibody, clone K65/35

Anti-Caspr Antibody, clone K65/35 is an antibody against Caspr for use in IH & WB.

Anti-Caspr Antibody, clone K65/35 MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Neuroscience Research Sub-Category: Signaling Neuroscience Gene Symbol: CNTNAP, Caspr1, caspr1, P190, p190, NRXN4, CASPR UniProt Number: P97846
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MABN69
100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      RIHC, WBMPurifiedMonoclonal Antibody
      Description
      Catalogue NumberMABN69
      DescriptionAnti-Caspr Antibody, clone K65/35
      Alternate Names
      • contactin associated protein 1
      • neurexin 4
      • neurexin-4
      • contactin-associated protein 1
      • Neurexin IV
      • Neurexin-4
      Background InformationContactin-associated protein 1(Caspr) is a single-pass type I membrane protein belonging to the neurexin family and is comprised of one F5/8 type C domain, one fibrinogen C-terminal domain, two EGF-like domains, and four laminin G-like domains. Caspr is well documented for its interaction with contactin, an interaction that is crucial to the transport of Caspr to the plasma membrane, the mylination of glial cells, and the generation of axoglial junctions. Studies also suggest that Caspr may play a regulatory role in the cell surface expression of contactin and its targeting to different axonal domains. Expression of Caspr is largely observed in the brain, mainly in CNS myelinated nerve fibers within paranodal axoglial junctions.
      References
      Product Information
      FormatPurified
      Control
      • Rat brain membrane tissue lysate
      PresentationPurified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Caspr Antibody, clone K65/35 is an antibody against Caspr for use in IH & WB.
      Key Applications
      • Immunohistochemistry
      • Western Blotting
      Application NotesImmunohistochemistry Analysis: 1:300 dilution from a previous lot detected Caspr in rat cerebellum and rat hippocampus tissues.
      Biological Information
      ImmunogenRecombinant protein corresponding to rat Caspr.
      CloneK65/35
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1κ
      Species Reactivity
      • Rat
      Species Reactivity NoteDemonstrated to react with rat. Predicted to react with mouse based on 100% sequence homology.
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryForms a complex with contactin; may mediate communication between glial cells and neurons during development [RGD].
      Gene Symbol
      • CNTNAP
      • Caspr1
      • caspr1
      • P190
      • p190
      • NRXN4
      • CASPR
      Purification MethodProtein G
      UniProt Number
      UniProt SummaryFUNCTION: Seems to play a role in the formation of functional distinct domains critical for saltatory conduction of nerve impulses in myelinated nerve fibers. Seems to demarcate the paranodal region of the axo-glial junction. In association with contactin may have a role in the signaling between axons and myelinating glial cells.

      SUBUNIT STRUCTURE: Interacts with contactin in cis form.

      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein (Potential).

      TISSUE SPECIFICTY: Predominantly expressed in brain. In myelinated nerve fibers of the CNS predominantly found in paranodal axoglial junctions. In unmyelinated nerve fibers of the CNS diffusely distributed along the entire surface. Weak expression is detected in ovary, pancreas, colon, lung, heart, intestine and testis.

      DEVELOPMENTAL STAGE: Detected on postnatal day 7 in cerebellum. Follows a caudorostral progession according to the myelination process. Appears to redistribute from the internode to the paranodal region during myelin compaction and maturation. Expression reaches maximal levels between days 14 and 18 and remains at the same levels until adulthood.

      SEQUENCE SIMILARITIES: Belongs to the neurexin family.

      Contains 2 EGF-like domains.

      Contains 1 F5/8 type C domain.

      Contains 1 fibrinogen C-terminal domain.

      Contains 4 laminin G-like domains.
      Molecular Weight~ 220 kDa observed
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blot in rat brain membrane tissue lysate.

      Western Blot Analysis: 0.5 µg/mL of this antibody detected Caspr on 10 µg of rat brain membrane tissue lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABN69 04053252413582

      Documentation

      Anti-Caspr Antibody, clone K65/35 SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Caspr Antibody, clone K65/35 Certificates of Analysis

      TitleLot Number
      Anti-Caspr, clone K65/35 2475913
      Anti-Caspr, clone K65/35 - 2165862 2165862
      Anti-Caspr, clone K65/35 - 2344734 2344734
      Anti-Caspr, clone K65/35 - 2391200 2391200
      Anti-Caspr, clone K65/35 - 1996248 1996248
      Anti-Caspr, clone K65/35 - 2199706 2199706
      Anti-Caspr, clone K65/35 - 2287237 2287237
      Anti-Caspr, clone K65/35 - 2562085 2562085
      Anti-Caspr, clone K65/35 - 3194639 3194639
      Anti-Caspr, clone K65/35 - 3445028 3445028
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