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AB3795 Anti-Cortactin Antibody, phospho-specific [Tyr466]

This Anti-Cortactin Antibody, phospho-specific [Tyr466] is validated for use in WB for the detection of Cortactin.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: CTTN, EMS1, Amplaxin, 1110020L01Rik, FLJ34459 UniProt Number: Q14247
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AB3795
100 µL  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3795
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Cortactin Antibody, phospho-specific [Tyr466]
      Alternate Names
      • Amplaxin
      • Oncogene EMS1
      Background InformationCortactin is cytoskeleton protein that facilitates assembly of cortical actin. Cortactin is widely expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src in vivo and in vitro. The protein sequence of cortactin contains multiple amino-terminal tandem repeats of a unique 37-amino acid sequence, which associates with actin, and a Src homology 3 domain at the carboxyl terminus.
      References
      Product Information
      FormatAffinity Purified
      PresentationAntibody is cross-adsorbed to nonphospho-peptide corresponding to the site of phosphorylation and preadsorbed with phosphopeptides to cortactin tyrosine 421 and 482 to remove cross-reactivity then affinity purified using phosphorylated cortactin (Tyr466) peptide. Antibody is supplied in phosphate-buffered saline (no Ca2+, no Mg2+), pH 7.3, containing 1 mg/ml BSA, and 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-Cortactin Antibody, phospho-specific [Tyr466] is validated for use in WB for the detection of Cortactin.
      Key Applications
      • Western Blotting
      Biological Information
      ImmunogenPhosphorylated cortactin (Tyr466) synthetic peptide corresponding to mouse amino acid residues around tyrosine 466 (tyrosine 470 in the human sequence).
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody detects the 80-85 kDa protein of cortactin.
      Species Reactivity
      • Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene is overexpressed in breast cancer and squamous cell carcinomas of the head and neck. The encoded protein is localized in the cytoplasm and in areas of the cell-substratum contacts. This gene has two roles: (1) regulating the interactions between components of adherens-type junctions and (2) organizing the cytoskeleton and cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. The aberrant regulation of this gene contributes to tumor cell invasion and metastasis. Two splice variants that encode different isoforms have been identified for this gene.
      Gene Symbol
      • CTTN
      • EMS1
      • Amplaxin
      • 1110020L01Rik
      • FLJ34459
      Modifications
      • Phosphorylation
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q14247 # May contribute to the organization of cell structure. The SH3 motif may function as a binding region to cytoskeleton. Tyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
      SIZE: 550 amino acids; 61636 Da
      SUBUNIT: Interacts with SHANK2 and SHANK3 via its SH2 domain. Also interacts with FGD1 (By similarity). Interacts with PLXDC2.
      SUBCELLULAR LOCATION: Cytoplasm (By similarity). Cell projection, lamellipodium (By similarity). Cell projection, ruffle (By similarity). Note=Associated with membrane ruffles and lamellipodia (By similarity).
      SIMILARITY: SwissProt: Q14247 ## Contains 7 cortactin repeats. & Contains 1 SH3 domain.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at -20°C for up to one year from date of receipt. Avoid repeated freeze/thaw cycles.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      AB3795 04053252740176

      Documentation

      Anti-Cortactin Antibody, phospho-specific [Tyr466] SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Cortactin Antibody, phospho-specific [Tyr466] Certificates of Analysis

      TitleLot Number
      RABBIT ANTI- PHOSPHO-EZRIN (THR567)/RADIXIN (THR564)/MOESIN (THR558) 2776253 2776253
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] 3072833
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] POLYCLONAL ANTIBODY - 2036672 2036672
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3190895 3190895
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3276073 3276073
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3755335 3755335
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3873520 3873520
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3887091 3887091
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3940293 3940293
      RABBIT ANTI-CORTACTIN PHOSPHO-SPECIFIC [TYR466] - 3984968 3984968
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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies