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MABT494 Anti-LIMS1, clone 5G7 Antibody

This Anti-LIMS1, clone 5G7 Antibody is validated for use in western blotting, ELISA, flow cytometry & immunofluorescence for the detection of LIMS1.

Anti-LIMS1, clone 5G7 Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: LIMS1, PINCH, PINCH1 UniProt Number: P48059
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MABT494
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HWB, ELISA, FC, IFMAscitesMonoclonal Antibody
      Description
      Catalogue NumberMABT494
      DescriptionAnti-LIMS1, clone 5G7 Antibody
      Alternate Names
      • LIM and senescent cell antigen-like-containing domain protein 1
      • Particularly interesting new Cys-His protein 1
      • PINCH-1
      • Renal carcinoma antigen NY-REN-48
      Background InformationLIM and senescent cell antigen-like-containing domain protein 1 (LIMS1), also known as Particularly interesting new Cys-His protein 1 (PINCH-1; PINCH1; PINCH), or Renal carcinoma antigen NY-REN-48 is an adaptor protein which contains five LIM domains, or double zinc fingers. LIMS1 is likely involved in integrin signaling through its LIM domain-mediated interaction with integrin-linked kinase, found in focal adhesion plaques. It is also thought to act as a bridge linking integrin-linked kinase to NCK adaptor protein 2, which is involved in growth factor receptor kinase signaling pathways. Its localization to the periphery of spreading cells also suggests that Lims1 may play a role in integrin-mediated cell adhesion or spreading.
      References
      Product Information
      FormatAscites
      PresentationMouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationThis Anti-LIMS1, clone 5G7 Antibody is validated for use in western blotting, ELISA, flow cytometry & immunofluorescence for the detection of LIMS1.
      Key Applications
      • Western Blotting
      • ELISA
      • Flow Cytometry
      • Immunofluorescence
      Application NotesELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).

      Flow Cytometry Analysis: A 1:200-400 dilution from a representative lot detected LIMS1 in HeLa cells.

      Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected LIMS1 in HepG2 cells.
      Biological Information
      ImmunogenPurified recombinant fragment of human LIMS1 expressed in E. Coli
      Clone5G7
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostMouse
      IsotypeIgG1
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Gene Symbol
      • LIMS1
      • PINCH
      • PINCH1
      Purification MethodUnpurified
      UniProt Number
      Molecular Weight~37 kDa observed. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in A549, Jurkat and HeLa cell lysates.

      Western Blotting Analysis: A 1:500-2,000 dilution of this antibody detected LIMS1 in A549, Jurkat and HeLa cell lysates.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at -20°C from date of receipt.
      Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      MABT494 04053252955488

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies