MAB3776
Anti-MDM2 Antibody, clone SMP14
clone SMP14, Chemicon®, from mouse
Synonym(s):
Murine Double Minute 2
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
SMP14, monoclonal
Application:
IHC (p), IP
Citations:
4
biological source
mouse
Quality Segment
conjugate
unconjugated
antibody form
purified immunoglobulin
antibody product type
primary antibodies
clone
SMP14, monoclonal
species reactivity
rat, human, mouse
manufacturer/tradename
Chemicon®
technique(s)
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable, immunoprecipitation (IP): suitable
isotype
IgG1
NCBI accession no.
UniProt accession no.
shipped in
wet ice
target post-translational modification
unmodified
Gene Information
human ... MDM2(4193)
mouse ... Mdm2(17246)
rat ... Mdm2(314856)
General description
MDM2 is over-expressed in many tumors. Its principal function is the ubiquitination and degradation of p53 tumour suppressor protein. This monoclonal antibody also recognizes a peptide epitope around Thr-216 of murine MDM2 when Thr-216 is unphosphorylated (Zhang & Prives, 2001). SMP14 also cross reacts with some cytokeratins (6, 14 & 16). This is only a problem when working with certain epithelial cells and not fibroblasts.
Immunogen
Synthetic peptide corresponding to residues 154-167 (CSRPSTSSRRRAISE) of human MDM2 cross-linked to KLH using glutaldehyde.
Application
Research Sub Category
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
Ubiquitin & Ubiquitin Metabolism
RNA Binding Protein (RBP)
Use Anti-MDM2 Antibody, clone SMP14 (Mouse Monoclonal Antibody) validated in IP, IHC, IHC(P) to detect MDM2 also known as Murine Double Minute 2.
Western blot
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Immunohistochemistry (Frozen and Paraffin-embedded sections)
Immunoprecipitation
ELISA
Optimal working dilutions must be determined by end user.
Biochem/physiol Actions
Recognizes human MDM2.
Physical form
Format: Purified
Liquid in 0.02M phosphate buffer, 0.25M NaCl, pH 7.6, with 0.1% sodium azide
Analysis Note
Control
POSITIVE CONTROL: Breast carcinoma
POSITIVE CONTROL: Breast carcinoma
Legal Information
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
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Storage Class
12 - Non Combustible Liquids
wgk
WGK 2
flash_point_f
Not applicable
flash_point_c
Not applicable
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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