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ABE1454 Anti-NR4A1 (Nur77)

Anti-NR4A1 (Nur77), Cat. No. ABE1454, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 1 and has been tested for use in Immunocytochemistry and Western Blotting.

Anti-NR4A1 (Nur77) MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Epigenetics & Nuclear Function Gene Symbol: NR4A1, GFRP1, HMR, NAK1 UniProt Number: P22736
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ABE1454
100 μg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Mk, M, HWB, ICCRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberABE1454
      DescriptionAnti-NR4A1 (Nur77)
      Alternate Names
      • Nuclear receptor subfamily 4 group A member 1
      • Early response protein NAK1
      • Nuclear hormone receptor NUR/77
      • Nur77
      • Orphan nuclear receptor HMR
      • Orphan nuclear receptor TR3
      • ST-59
      • Testicular receptor 3
      Background InformationNuclear receptor subfamily 4 group A member 1 (UniProt: P22736; also known as Early response protein NAK1, Nuclear hormone receptor NUR/77, Nur77, Orphan nuclear receptor HMR, Orphan nuclear receptor TR3, ST-59, Testicular receptor 3) is encoded by the NR4A1 (also known as GFRP1, HMR, NAK1) gene (Gene ID: 3164) in human. Nur77 is an orphan nuclear receptor that may act concomitantly with NURR1 in regulating the expression of delayed-early genes during liver regeneration. It is shown to be expressed in fetal muscle and in adult liver, brain, and thyroid and is reported to be induced by growth stimulating agents. It binds the NGFI-B response element (NBRE) 5'-AAAAGGTCA-3'. Its DNA binding region is localized to amino acids 264-339. It contains two zinc finger domains (aa 267-287 and 303-327). Nur77 is shown to heterodimerize with retinoid X receptor alpha and is actively transported from the nucleus to the mitochondria in cells undergoing apoptosis. It binds to Bcl-2 and triggers a conformational change that exposes the pro-apoptotic BH3 domain. This converts Bcl-2 from an anti-apoptotic to a pro-apoptotic factor. In several cancers the nuclear-mitochondrial translocation of Nur77/TR3 is defective, which prevents apoptosis. Three isoforms of Nur77 have been described that are produced by alternative splicing. It is acetylated by p300/CBP that increases its stability. It can also undergo phosphorylation at Serine 351 by RPS6KA1 and RPS6KA3 in response to mitogenic or stress stimuli.
      References
      Product Information
      FormatAffinity Purified
      PresentationPurified rabbit polyclonal antibody in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-NR4A1 (Nur77), Cat. No. ABE1454, is a highly specific rabbit polyclonal antibody that targets Nuclear receptor subfamily 4 group A member 1 and has been tested for use in Immunocytochemistry and Western Blotting.
      Key Applications
      • Western Blotting
      • Immunocytochemistry
      Application NotesWestern Blotting Analysis: 2 µg/mL from a representative lot detected NR4A1 (Nur77) in 10 µg of COS7 cell lysate.

      Immunocytochemistry Analysis: A 1:100 dilution from a representative lot detected NR4A1 (Nur77) in HeLa, A431, HUVEC, and NIH/3T3 cells.
      Biological Information
      ImmunogenKLH-conjugated linear peptide corresponding to 17 amino acids from the N-terminal region of human Nuclear receptor subfamily 4 group A member 1 (NR4A1). The immunogen sequence is conserved in all 3 isoforms.
      ConcentrationPlease refer to lot specific datasheet.
      HostRabbit
      SpecificityThis rabbit polyclonal antibody detects Nuclear receptor subfamily 4 group A member 1 (NR4A1) in human, Monkey, and Murine cells It targets an epitope within 17 amino acids from the N-terminal region.
      Species Reactivity
      • Monkey
      • Mouse
      • Human
      Species Reactivity NoteHuman, Monkey, Mouse
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • NR4A1
      • GFRP1
      • HMR
      • NAK1
      Purification MethodAffinity Purified
      UniProt Number
      Molecular Weight~64 kDa observed; 64.46 kDa calculated. Uncharacterized bands may be observed in some lysate(s).
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Western Blotting in HeLa cell lysate.

      Western Blotting Analysis: 2 µg/mL of this antibody detected NR4A1 (Nur77) in 10 µg of HeLa cell lysate.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 μg
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      ABE1454 04054839416538