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05-829-AF647 Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate

Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.

MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Cell Structure Research Sub-Category: Cytoskeleton Gene Symbol: TUBA1C, TUBA1 UniProt Number: P02552
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05-829-AF647
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      M, H, Gp, Av, B, Porcine, Gr, RICCMAlexaFluor®647Monoclonal Antibody
      Description
      Catalogue Number05-829-AF647
      DescriptionAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate
      Alternate Names
      • Tubulin alpha-1 chain
      • α-Tubulin
      Background InformationTubulin alpha-1 chain (UniProt P02552) is encoded by the TUBA1C (also known as TUBA1) gene (Gene ID 429035) in Gallus gallus (Chicken) species. Microtubules are cytoskeletal filaments composed of alpha-/beta-tubulin heterodimers that self-assemble head-to-tail to form protofilaments and laterally to form a hollow tube. Various post-translational modifications, including acetylation, polyglutamylation, polyglycylation, detyrosination, phosphorylation, and palmitoylation, can regulate the polymerization properties of tubulins and/or their interactions with microtubule associated proteins (MAPs) and motor proteins.
      References
      Product Information
      FormatAlexaFluor®647
      PresentationPurified mouse monoclonal IgG1 antibody conjugate in PBS with 15 mg/mL BSA and 0.1 % sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate is an antibody against α-Tubulin for use in Immunocytochemistry.
      Key Applications
      • Immunocytochemistry
      Application NotesImmunocytochemistry Analysis: A 1:100 dilution from a representative lot detected α-Tubulin in NIH/3T3 cells.
      The unconjugated antibody (Cat. No. 05-829) is shown to be suitable also for immunocytochemistry, immunofluorescence, and Western blotting applications.
      Biological Information
      ImmunogenPurified chicken brain α-Tubulin. The epitope recognized by this antibody has been mapped to the C-terminal 25 amino acids (AALEKDYEEVGVDSVEGEGEEEGEE).
      EpitopeC-terminal 25 amino acids.
      CloneDM1A
      ConcentrationPlease refer to lot specific datasheet.
      HostMouse
      SpecificityClone DM1A recognizes α-Tubulin, MW 50-60 kDa. The epitope recognized by clone DM1A has been mapped to the C-terminal 25 amino acids.
      IsotypeIgG1
      Species Reactivity
      • Mouse
      • Human
      • Guinea Pig
      • Avian
      • Bovine
      • Porcine
      • Gerbil
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TUBA1C
      • TUBA1
      Purification MethodProtein G Purified
      UniProt Number
      Molecular Weight~50 kDa observed.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceEvaluated by Immunocytochemistry in A431 cells.

      Immunocytochemistry Analysis: A 1:100 dilution of this antibody detected α-Tubulin in A431 cells.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      05-829-AF647 04055977330571

      Documentation

      Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-α-Tubulin Antibody, clone DM1A, Alexa Fluor® 647 conjugate Certificates of Analysis

      TitleLot Number
      Anti-α-Tubulin, clone DM1A, -Q2662538 Q2662538
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3217766 3217766
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3539741 3539741
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3810421 3810421
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 3852427 3852427
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 4093602 4093602
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 4181448 4181448
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 4229037 4229037
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate - 4311822 4311822
      Anti-α-Tubulin, clone DM1A, Alexa Fluor® 647 conjugate Monoclonal Antibody 3060026
      Show more

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies