Anti-WHSC1/NSD2 Antibody, clone 29D1

Wolf-Hirschhorn syndrome candidate 1 protein (WHSC1), Nuclear SET domain containing protein 2 (NSD2) is a histone lysine methyltransferase that is deregulated in a subgroup of multiple myelomas. NSD proteins have been described to serve as oncogenes; however, little is known about their targets in transcriptional repression or activation. NSD2 is overexpressed in multiple myelomas via translocation between the NSD2 locus on chromosome 4 and the immunoglobulin locus on chromosome 14. NSD2 is under the regulatory control of this immunoglobulin locus. Further research is required to elucidate its roles and mechanisms in cancer.

~152 kDa observed. There are seven isoforms ranging between ~30 and ~152 kDa Different lysates may indicate different isoforms in Western Blots.

GST-tagged recombinant protein corresponding to human WHSC1/NSD2.

Detect WHSC1/NSD2 using this Anti-WHSC1/NSD2 Antibody, clone 29D1 validated for use in Western Blotting, Chromatin Immunoprecipitation (ChIP), Immunoprecipitation.

Western Blot Analysis: 0.5-1 µg/mL from a representative lot detected WHSC1/NSD2 in 10 µg of HeLa nuclear extract and human placenta tissue lysate.

Chromatin Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in transfected Gal4-reporter/293T cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Immunoprecipitation Analysis: A representative lot was used by an independent laboratory to detect WHSC1/NSD2 in KMS11 cells. (Marango, J., et al. (2008). Blood. 111:3145-3154.)

Format: Purified

Evaluated by Western Blot in HCT116 cell lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected WHSC1/NSD2 in 10 µg of HCT116 cell lysate.

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.