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07-838 Anti-phospho-IR (Tyr972) Antibody

Anti-phospho-IR (Tyr972) Antibody detects level of phospho-IR (Tyr972) & has been published & validated for use in WB.

Anti-phospho-IR (Tyr972) Antibody MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.
Research Category: Signaling Research Sub-Category: Insulin/Energy Signaling Gene Symbol: INSR, CD220, HHF5, IR UniProt Number: P06213
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07-838
100 µL  
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      Overview

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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      H, MWBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue Number07-838
      Brand Family Upstate
      Trade Name
      • Upstate
      DescriptionAnti-phospho-IR (Tyr972) Antibody
      Alternate Names
      • Insulin Receptor
      • CD220
      References
      Product Information
      FormatAffinity Purified
      Presentation100 μL of affinity purified rabbit polyclonal IgG in 50% storage buffer (PBS (without Mg2+ and Ca2+), pH 7.3 containing 1.0 mg/mL BSA (IgG and protease free) and 0.05% sodium azide) and 50% glycerol.
      Quality LevelMQ100
      Applications
      ApplicationAnti-phospho-IR (Tyr972) Antibody detects level of phospho-IR (Tyr972) & has been published & validated for use in WB.
      Key Applications
      • Western Blotting
      Biological Information
      Immunogen A linear peptide corresponding to amino acid region encompassing the human, mouse and rat phospho-IR (pTyr972).
      HostRabbit
      SpecificityRecognizes phospho-IR (β-subunit) (pTyr972).
      IsotypeIgG
      Species Reactivity
      • Human
      • Mouse
      Species Reactivity NotePredicted to cross-react with rat based on sequence homology (100%), but have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryAfter removal of the precursor signal peptide, the insulin receptor precursor is post-translationally cleaved into two chains (alpha and beta) that are covalently linked. Binding of insulin to the insulin receptor (INSR) stimulates glucose uptake. Two transcript variants encoding different isoforms have been found for this gene.
      Gene Symbol
      • INSR
      • CD220
      • HHF5
      • IR
      Modifications
      • Phosphorylation
      Purification MethodAffinity Purfied
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P06213 # This receptor binds insulin and has a tyrosine-protein kinase activity. Isoform Short has a higher affinity for insulin. Mediates the metabolic functions of insulin. Binding to insulin stimulates association of the receptor with downstream mediators including IRS1 and phosphatidylinositol 3'-kinase (PI3K). Can activate PI3K either directly by binding to the p85 regulatory subunit, or indirectly via IRS1.
      SIZE: 1382 amino acids; 156307 Da
      SUBUNIT: Tetramer of 2 alpha and 2 beta chains linked by disulfide bonds. The alpha chains contribute to the formation of the ligand- binding domain, while the beta chains carry the kinase domain. Interacts with SORBS1 but dissociates from it following insulin stimulation. Binds SH2B2. Interacts with the PTB/PID domains of IRS1 and SHC1 in vitro when autophosphorylated on tyrosine residues. The sequences surrounding the phosphorylated NPXY motif contribute differentially to either IRS1 or SHC1 recognition. Interacts with the SH2 domains of the 85 kDa regulatory subunit of PI3K (PIK3R1) in vitro, when autophosphorylated on tyrosine residues. Interacts with SOCS7.
      SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
      TISSUE SPECIFICITY: Isoform Long and isoform Short are expressed in the peripheral nerve, kidney, liver, striated muscle, fibroblasts and skin. Isoform Short is expressed also in the spleen and lymphoblasts.
      PTM: After being transported from the endoplasmic reticulum to the Golgi apparatus, the single glycosylated precursor is further glycosylated and then cleaved, followed by its transport to the plasma membrane. & Autophosphorylated on tyrosine residues in response to insulin. & Phosphorylation of Tyr-999 is required for IRS1- and SHC1- binding.
      DISEASE: SwissProt: P06213 # Defects in INSR are the cause of insulin resistance (Ins resistance) [MIM:125853]. & Defects in INSR are the cause of Rabson-Mendenhall syndrome [MIM:262190]; also known as Mendenhall syndrome. It is a severe insulin resistance syndrome characterized by insulin- resistant diabetes mellitus with pineal hyperplasia and somatic abnormalities. Typical features include coarse, senile-appearing facies, dental and skin abnormalities, abdominal distension, and phallic enlargement. Inheritance is autosomal recessive. & Defects in INSR are the cause of leprechaunism [MIM:246200]; also known as Donohue syndrome. Leprechaunism represents the most severe form of insulin resistance syndrome, characterized by intrauterine and postnatal growth retardation and death in early infancy. Inheritance is autosomal recessive. & Defects in INSR may be associated with noninsulin- dependent diabetes mellitus (NIDDM) [MIM:125853]; also known as diabetes mellitus type 2. & Defects in INSR are the cause of familial hyperinsulinemic hypoglycemia 5 (HHF5) [MIM:609968]. Familial hyperinsulinemic hypoglycemia [MIM:256450], also referred to as congenital hyperinsulinism, nesidioblastosis, or persistent hyperinsulinemic hypoglycemia of infancy (PPHI), is the most common cause of persistent hypoglycemia in infancy and is due to defective negative feedback regulation of insulin secretion by low glucose levels. & Defects in INSR are the cause of insulin-resistant diabetes mellitus with acanthosis nigricans type A (IRAN type A) [MIM:610549]. This syndrome is characterized by the association of severe insulin resistance (manifested by marked hyperinsulinemia and a failure to respond to exogenous insulin) with the skin lesion acanthosis nigricans and ovarian hyperandrogenism in adolescent female subjects. Women frequently present with hirsutism, acne, amenorrhea or oligomenorrhea, and virilization. This syndrome is different from the type B that has been demonstrated to be secondary to the presence of circulating autoantibodies against the insulin receptor.
      SIMILARITY: SwissProt: P06213 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. Insulin receptor subfamily. & Contains 2 fibronectin type-III domains. & Contains 1 protein kinase domain.
      Molecular Weight155 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 2 years at -20°C from date of shipment.
      Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      07-838 04053252622496

      Documentation

      Required Licenses

      Title
      PRODUCTO REGULADO POR LA SECRETARÍA DE SALUD

      Anti-phospho-IR (Tyr972) Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-phospho-IR (Tyr972) Antibody Certificates of Analysis

      TitleLot Number
      Anti-phospho-IR (Tyr972) (Affinity purified Rabbit polyclonal IgG) 2981108A
      Anti-phospho-IR (Tyr972) (Affinity purified Rabbit polyclonal IgG) 3082608
      Anti-phospho-IR (Tyr972) - 3385875 3385875
      Anti-phospho-IR (Tyr972) - 3447531 3447531
      Anti-phospho-IR (Tyr972) - 3455143 3455143
      Anti-phospho-IR (Tyr972) - 3839126 3839126
      Anti-phospho-IR (Tyr972) - 3892562 3892562
      Anti-phospho-IR (Tyr972) - 3947083 3947083
      Anti-phospho-IR (Tyr972) - 4018502 4018502
      Anti-phospho-IR (Tyr972) - 4021660 4021660
      Show more

      References

      Reference overviewPub Med ID
      Preparation, crystallization and preliminary X-ray analysis of threonine synthase from Streptococcus mutans.
      De-Wei Tang,Lan-Fen Li,Ya-Mei Yu,Xiang-Yu Liu,Xiao-Dong Su,Xiaojun Zhao,Yu-He Liang
      Protein and peptide letters  14  2007

      Show Abstract       		17979829 17979829

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      Categories

      Life Science Research > Antibodies and Assays > Primary Antibodies