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1.15940

Crystal violet (C.I. 42555)

Name: Crystal violet (C.I. 42555)
Brand: MM

for microscopy Certistain^®

Synonym(s):

Crystal violet (C.I. 42555), Hexamethylenepararosaniline chloride, Gentian violet (C.I. 42555), Hexamethyl-p-rosanilinium, Methyl violet 10 B

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About This Item

Empirical Formula (Hill Notation):

C₂₅H₃₀ClN₃

CAS Number:

548-62-9

Molecular Weight:

407.98

EC Index Number:

208-953-6

UNSPSC Code:

41116130

MDL number:

MFCD00011750

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Properties

Quality Segment

400

form

solid

potency

420 mg/kg LD₅₀, oral (Rat)

composition

Dye, ≥88% spectrophotometric assay

pH

2.5-3.5 (20 °C, 10 g/L in H₂O)

mp

189-194 °C

solubility

10 g/L

density

1.19 g/cm³ at 20 °C

bulk density

220‑400 kg/m³

ε_max

2050-2450 at 589-594 nm in water at 0.002 g/L

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

2-30°C

InChI

1S/C25H30N3.ClH/c1-26(2)22-13-7-19(8-14-22)25(20-9-15-23(16-10-20)27(3)4)21-11-17-24(18-12-21)28(5)6;/h7-18H,1-6H3;1H/q+1;/p-1

InChI key

ZXJXZNDDNMQXFV-UHFFFAOYSA-M

Description

General description

Crystal violet (C.I. 42555) for microscopy Certistain^®, is a dry dye that can be used to produce a Crystal violet staining solution to differentiate Gram-positive and Gram-negative bacteria in samples of human origin. In the case of Gram-positive bacteria, the bacteria retain their dark blue / dark purple color even after the treatment with decolorizing solution. On the other side Gram-negative bacteria are stained red or pink with the counterstain. The Certistain^® dry dye can be used for clinical diagnostic purposes as it is certified and registered as IVD and CE product. For more details, please see instructions for use (IFU). The IFU can be downloaded from this webpage.

Analysis Note

Dye content (spectrophotometrically): ≥ 88%
Identity (UV/VIS-Spectrum): passes test
Absorption maximum λ max (water): 589 - 594 nm
Spec. Absorptivity A 1%/1cm (λ_m_a_x; 0.002 g/l; water): 2050 - 2450
TLC-Test: passes test
Michler`s ketone: <0.10%
Loss on drying (110 °C): ≤ 10%
Suitability for microscopy: passes test

Legal Information

CERTISTAIN is a registered trademark of Merck KGaA, Darmstadt, Germany

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Danger

Hazard Classifications

Acute Tox. 4 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - Carc. 2 - Eye Dam. 1

Storage Class

11 - Combustible Solids

wgk

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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