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MCKP1-110K MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1

The Mouse Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 28 immune checkpoint proteins in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.

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MCKP1-110K
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Overview

Replacement Information
Description
Catalogue NumberMCKP1-110K
Trade Name
  • MILLIPLEX
DescriptionMILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1
OverviewEMD Millipore’s MILLIPLEX® Mouse Immuno-Oncology Checkpoint Protein Panel 1 is a 28-plex kit to be used for the simultaneous quantification of any or all of the following analytes in serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples: 4-1BBL/TNFSF9, 5’-NT/CD73, B7-H2/ICOSL, B7-H3/CD276, BCA-1/CXCL-13, BTLA, CD137/4-1BB, CD226/DNAM-1, CD25/IL-2Rα, CD27, CD40, CD80/B7-1, CTLA-4/CD152, E-cadherin, Galectin-1, Galectin-3, GITR, Granzyme B, HVEM, IFNγ, IL-10, LAG-3, PD-1, PD-L1, PD-L2, TIM-3, TLR-2, and TNFα.
Background InformationImmune checkpoint proteins are key regulators of the immune system and drug targets of cancer immunotherapy. Immune checkpoint molecules have been found on the cell surface of immune cells and tumor cells as activating receptors or inhibitory receptors. Immune checkpoint inhibitors, such as blocking antibodies to cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), programmed cell death protein-1 (PD-1) and programmed cell death ligand 1 (PD-L1), have demonstrated significant efficacy in the treatment of a variety of cancers. Recent studies have also shown many immune checkpoint proteins can undergo alternative splicing or proteolytic cleavage to produce soluble checkpoint proteins. The expression levels of soluble and cell-surface immune checkpoint proteins in the circulation and in tumor-microenvironment (TME) may be used as putative immune-based biomarkers for cancer research and biomarker discovery in immunological diseases.
References
Product Information
Detection methodLuminex xMAP
ConfigurationDesign your multiplex kit by choosing available analytes within this panel.
Precision, %
  • Inter-assay: <20
  • Intra-assay: <10
Panel TypeMAGNETIC Circulating Cancer
Quality LevelMQ200
Applications
ApplicationThe Mouse Immuno-Oncology Checkpoint Protein Panel 1 bead-based multiplex panel, using the Luminex xMAP technology, enables the simultaneous analysis of 28 immune checkpoint proteins in mouse serum, plasma, cell culture supernatant, cell lysate, and tissue/tumor extract samples.
Key Applications
  • Multiplexing
Application NotesThis is an overnight incubation assay (16-18 hours at 2-8°C).
A maximum of 25 μL per well 1:2 diluted serum or plasma can be used. Tissue culture samples may be run neat or appropriately diluted in control medium. Note: Best results using cell/tissue lysate samples may require diluting in assay buffer while testing similarly diluted lysis buffer for matrix effects.
Biological Information
SpecificityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Species Reactivity
  • Mouse
Analytes Available
  • 4-1BBL/TNFSF9
  • B7-H2/ICOSL
  • B7-H3/CD276
  • 5'-NT/CD73
  • CD137/4-1BB
  • CD226/DNAM-1
  • E-Cadherin
  • BCA-1
  • BTLA
  • CD27
  • CD80/B7-1
  • CTLA-4
  • CD40
  • Galectin-1 (GAL1)
  • Galectin-3
  • GITR
  • Granzyme B
  • HVEM
  • IFNγ
  • IL-10
  • LAG-3
  • PD-1
  • PD-L1
  • PD-L2
  • TIM-3
  • TLR-2
  • TNFα
  • CD25/IL-2Rα
Cross ReactivityCross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Accuracy
  • BTLA: 111%
  • 4-1BBL/TNFSF9: 105%
  • CD137/4-1BB: 107%
  • B7-H2/ICOSL: 110%
  • B7-H3/CD276: 95%
  • 5’-NT/CD73: 106%
  • BCA-1/CXCL-13: 105%
  • CD226/DNAM-1: 110%
  • CD25/IL-2Rα: 105%
  • CD27: 107%
  • CD40: 109%
  • CD80/B7-1: 103%
  • CTLA-4/CD152: 104%
  • E-Cadherin: 108%
  • Galectin-1: 101%
  • Galectin-3: 108%
  • GITR: 109%
  • Granzyme B: 94%
  • HVEM: 108%
  • IFNγ: 103%
  • IL-10: 105%
  • LAG-3: 105%
  • PD-1 : 106%
  • PD-L1: 96%
  • PD-L2: 104%
  • TIM-3: 106%
  • TLR-2: 104%
  • TNFα: 106%
Physicochemical Information
Sensitivity
  • Refer to kit protocol for sensitives for individual analytes.
Standard Curve Range
  • 4-1BBL/TNFSF9: 14 – 10,000 pg/mL
  • 5’-NT/CD73: 137 – 100,000 pg/mL
  • B7-H2/ICOSL: 27 – 20,000 pg/mL
  • B7-H3/CD276: 3.4 – 2,500 ng/mL
  • BCA-1/CXCL-13: 27 – 20,000 pg/mL
  • BTLA: 21 – 15,000 pg/mL
  • CD137/4-1BB: 5.5 – 4,000 pg/mL
  • CD226/DNAM-1: 14 – 10,000 pg/mL
  • CD25/IL-2Rα: 2.7 – 2,000 pg/mL
  • CD27: 55 – 40,000 pg/mL
  • CD40: 14 – 10,000 pg/mL
  • CD80/B7-1: 34.3 – 25,000 pg/mL
  • CTLA-4/CD152: 5.5 – 4,000 pg/mL
  • E-Cadherin: 342 – 250,000 pg/mL
  • Galectin-1: 3.4 – 2,500 ng/mL
  • Galectin-3: 41 – 30,000 pg/mL
  • GITR: 41 – 30,000 pg/mL
  • Granzyme B: 8.2 – 6,000 pg/mL
  • HVEM: 1.4 – 1,000 pg/mL
  • IFNγ: 2.1 – 1,500 pg/mL
  • IL-10: 11 – 8,000 pg/mL
  • LAG-3: 4.12 – 300,000 pg/mL
  • PD-1: 55 – 40,000 pg/mL
  • PD-L1: 14 – 10,000 pg/mL
  • PD-L2: 6.9 - 5,000 pg/mL
  • TIM-3: 5.5 - 4,000 pg/mL
  • TLR-2: 412 - 3,000 pg/mL
  • TNFα: 4.1 - 3,000 pg/mL
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Usage Statement
  • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage and Shipping Information
Storage ConditionsRecommended storage for kit components is 2 - 8°C.
Packaging Information
Material Size96-well plate
Material Package96 well plate
Transport Information
Supplemental Information
Specifications
Global Trade Item Number
Catalogue Number GTIN
MCKP1-110K 04065267298702

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