MABS814
Anti-MAP2K4, clone 5H4 Antibody
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About This Item
UNSPSC Code:
12352203
NACRES:
NA.41
eCl@ss:
32160702
Conjugate:
unconjugated
Clone:
5H4, monoclonal
Application:
ELISA, FACS, IHC, WB
Citations:
-
conjugate
unconjugated
Quality Segment
antibody form
ascites fluid
clone
5H4, monoclonal
purified by
(unpurified)
species reactivity
human, mouse
concentration
(Please refer to lot specific datasheet.)
technique(s)
ELISA: suitable, flow cytometry: suitable, immunohistochemistry: suitable, western blot: suitable
isotype
IgG1
UniProt accession no.
target post-translational modification
unmodified
General description
Purified recombinant fragment of human MAP2K4 expressed in E. Coli.
~40 kDa observed. Uncharacterized bands may be observed in some lysate(s).
Application
Immunohistochemistry Analysis (IHC): A 1:200-1,000 dilution from a representative lot detected MAP2K4 in muscle tissue.
Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K4 in K562 cells.
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Flow Cytometry Analysis (FC): A 1:200-400 dilution from a representative lot detected MAP2K4 in K562 cells.
ELISA: A representative lot detected control antigen (100 ng) and antigen (10 ng, 50 ng, 100 ng) in ELISA (Direct).
Physical form
Mouse monoclonal IgG1 ascitic fluid containing 0.03% sodium azide.
Preparation Note
Stable for 1 year at -20°C from date of receipt.Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Analysis Note
Evaluated by Western Blotting in HepG2, K562 and HEK293 cell lysates.
Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K4 in HepG2, K562 and HEK293 cell lysates.
Western Blotting Analysis (WB): A 1:500-2,000 dilution of this antibody detected MAP2K4 in HepG2, K562 and HEK293 cell lysates.
Disclaimer
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Storage Class
10 - Combustible liquids
wgk
WGK 1
Certificates of Analysis (COA)
Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.
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