Skip to Content
Merck

Key Documents

View All Documentation

S9305

SYBR™ Green II RNA gel stain

greener alternative

Greener Alternative

10,000 × in DMSO

Synonym(s):

RNA gel dye, safer gel stain, SYBR™ RNA dye

Sign In to View Organizational & Contract Pricing.

Select a Size

Change View

About This Item

CAS Number:
172827-25-7
UNSPSC Code:
12171500
EC Number:
200-664-3
NACRES:
NA.52
MDL number:
MFCD00284716
Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

usage

 mL sufficient for 100 mini-gels

Quality Segment

100

greener alternative product characteristics

Designing Safer Chemicals
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

concentration

10,000 × in DMSO

greener alternative category

Aligned,

storage temp.

−20°C

General description

SYBR™ Green II is a highly sensitive stain for post electrophoresis staining of RNA and ssDNA in agarose or polyacrylamide gels. SYBR™ Green II is not selective for RNA staining but does exhibit a higher quantum yield when bound to RNA than to double stranded DNA.
Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has inherently safer chemistry, compared to the standard use of ethidium bromide for staining. For more information see publication found in related content.

Application

SYBR™ Green II RNA gel stain has been used:

  • for the quantification of ribonucleic acid (RNA) in Escherichia coli
  • for staining 5′-ETS rRNA species separated on polyacrylamide gel prior to Northern blot analysis
  • to stain formaldehyde agarose gels to visualize RNA bands

Features and Benefits

  • Ultrasensitive stain for post-electrophoresis staining of RNA and ssDNA
  • Excited maximally at 497 nm with a secondary excitation peak at 254nm
  • The fluorescence emission of SYBR™Green II stained RNA is centered at 520 nm
  • Staining agarose/formaldehyde gels with SYBR Green II does not interfere with the transfer of RNA to membranes or subsequent hybridization in Northern blot analysis as long as 0.1% -0.3% SDS is included in prehybridization and hybridization buffers to remove the dye
  • Might facilitate the detection of viroid RNAs and multicopy cellular RNA species
  • More sensitive than ethidium bromide in applications that require extremely sensitive detection techniques such as single-strand conformation polymorphism (SSCP) analysis

Legal Information

SYBR is a trademark of Thermo Fisher Scientific or its subsidiaries

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)

Storage Class

10 - Combustible liquids

flash_point_f

188.6 °F - closed cup

flash_point_c

87 °C - closed cup

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Search for a COA

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library