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Merck

CMC0008

STEADY Chemically Competent Cells

Escherichia coli, rod shaped

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UNSPSC Code:
12352200
Biological source:
Escherichia coli
Growth mode:
adherent or suspension
Morphology:
rod shaped
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補品名

STEADY Chemically Competent Cells, for plasmid production using unstable DNA

biological source

Escherichia coli

grade

Molecular Biology

form

buffered aqueous solution

growth mode

adherent or suspension

morphology

rod shaped

technique(s)

microbiological culture: suitable

cell transformation

competent cell type: chemically competent
transformation efficiency: >1 x 108 cfu/ÎŒg

shipped in

dry ice

storage temp.

−70°C

General description

The STEADY Chemically Competent Cells are a commonly used strain for cloning sequences that suffer unwanted recombination events in other strains.
Clones with inverted repeats or other sequnces prone to recombination are commonly found in retroviral genes, and require cells such as this product to be propagated stably.

Genotype

recA13 supE44 ara-14 galK2 lacY1 proA2 rpsL20(StrR) xyl-5 λ– leu mtl-1 F– mcrB mrr hsdS20(rB–, mB–)

Features and Benefits

  • Clone unstable sequences with reliable results
  • Available as chemically competent or electrocompetent cells
  • Your choice of two packaging formats
  • High efficiency: over 1 × 108 cfu/ÎŒg (chemi) or 1 × 1010 cfu/ÎŒg (electro)
  • Excellent value

Other Notes

  • STEADY chemically competent cells
  • pUC 19 transformation control DNA
  • recovery medium for cloning




保管分類

10 - Combustible liquids


適甚法什

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pdsc

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prtr

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fsl

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ishl_indicated

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ishl_notified

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