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Merck

SML4395

BODIPY-FL-C12

≥98% (HPLC)

Synonym(s):

BODIPY 505/515 C12 Green, BODIPY-C12 Green, BDY-C12, [5-[(3,5-Dimethyl-2H-pyrrol-2-ylidene-κN)methyl]-1H-pyrrole-2-dodecanoato(2-)-κN1]difluoro-borate(1-)

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About This Item

Empirical Formula (Hill Notation):
C23H33BF2N2O2
CAS Number:
Molecular Weight:
418.33
UNSPSC Code:
12352200
Assay:
≥98% (HPLC)
Form:
powder
Storage condition:
protect from light
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Quality Segment

assay

≥98% (HPLC)

form

powder

storage condition

protect from light

color

red to very dark purple

solubility

DMSO: 2 mg/mL, clear

storage temp.

-10 to -25°C

Biochem/physiol Actions

BODIPY-FL-C12 is a fluorescent fatty acid analog, resemble the length of C16 FA such as palmitic acid, designed for real-time visualization of lipid metabolism in living cells. Structurally, a C12 alkyl chain directs the molecule into cellular fatty acid transport pathways, while the core BODIPY fluorophore provides exceptionally bright and photostable green fluorescence (λex/λem ≈ 505/515 nm). Following rapid cellular uptake, BODIPY-FL-C12 is esterified by acyl-CoA synthetase in the endoplasmic reticulum and subsequently trafficked for robust, irreversible accumulation within the neutral lipid core of lipid droplets. This metabolic trapping mechanism generates a high signal-to-noise ratio, enabling high-resolution tracking of fatty acid uptake, transport, and storage dynamics. Its proven utility in live-cell imaging, flow cytometry, and in vivo models like C. elegans supplants traditional endpoint assays. BODIPY-FL-C12′s low cytotoxicity and high cell permeability make it an essential tool for dynamically investigating metabolic regulation in obesity, diabetes, and cancer research.


Storage Class

11 - Combustible Solids

flash_point_f

Not applicable

flash_point_c

Not applicable



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