MultiScreen® Filter Plates
MultiScreen® plates with 96- and 384-well capacities are available in many configurations and a diverse set of housing materials to assure compatibility with varied media formulations and solvents. MultiScreen® Solid Bottom Plates are available for chemiluminescent and fluorescent screening assays, sample preparation and storage, and ELISA including 8-well strip format. Filter plates are invaluable for sample preparation including cell separation for flow cytometry, ELISpot, cell migration, invasion and chemotaxis, enzyme and receptor ligand binding, solubility and permeability assays, including PAMPA. Plates are automation compatible and designed for use with DirectStack™ vacuum workflow to minimize sample cross-talk. Product customization is available including barcodes, membrane selection, and packaging.
Automation Workflow using MultiScreen® Filter Plates
MultiScreen® filter plates were designed to work productively with sensitive detection and imaging systems, providing dependable and robust sample handling and precision transfer. Plates with 96- and 384-well capacity are fabricated using a diverse range of housing materials to ensure compatibility with various sample types. MultiScreen® filtration plates are offered in a broad selection of membrane filters with both sterile and non-sterile options.
- Durapore® (PVDF) membranes: Low protein- and nucleic acid-binding membranes in 0.22 - 1.2 µm pore sizes for sample preparation, biochemical assays, and bead-based applications
- Immobilon®P (PVDF) membranes: High protein and nucleic acid-binding membranes available in 0.45 µm pore size for ELISpot including fluorescent ELISpot and DNA or protein binding assays
- Various other membranes are available including MCE (Nitrocellulose), PTFE, Polycarbonate, and more
MultiScreen®HTS filter plates
MultiScreen®HTS plates are specifically designed for high throughput use with automated workstations. Firm sidewalls improve handling, and ample surfaces are provided for barcode label placement. Individual wells are sealed to prevent crosstalk arising from incubation. The patented underdrain is removable to provide easy access to the filters. MultiScreen®HTS+ filter plates have a unique, nylon mesh backing for enhanced washing, reducing background and improving signal-to-noise ratios. Filter plates are available in 96-and 384-well formats, and are available in a broad selection of membranes and plastics.
MultiScreen® Classic filter plates
MultiScreen® Classic plates are specifically designed for assays requiring removal of the underdrain and access to membranes. Flexible plate base material can be removed by hand for low throughput assays. Individual wells are sealed to prevent crosstalk arising from incubation. Filter plates are available in 96-well format and are available in a broad selection of membranes and plastics.
MultiScreen® Solvinert™ filter plates
For sample preparation, protein precipitation with solvents, bead cleavage assays, and quantitative filtrate transfer for analysis, we recommend MultiScreen® Solvinert™ filter plates. These plates contain hydrophilic or hydrophobic PTFE membranes which ensure low extractables, low undesirable binding, and high analyte recovery.
MultiScreen® MESH filter plates
MultiScreen® MESH systems use a 96-well nylon mesh-bottom filter plate, and constitute a complete system for target screening and other critical applications by evaluating new compounds using multicellular organisms as the in vivo model. These systems are routinely used in pharmaceutical and agropharma discovery for assays measuring cytotoxicity, paralysis, and death. Plates are constructed of optically-clear plastic to facilitate imaging.
MultiScreen®-MIC filter plates for migration, invasion and chemotaxis
The MultiScreen®-MIC filter plate provides a reliable, versatile platform for a range of cell-based screening assays including migration, invasion, chemotaxis, co-culture, angiogenesis, and transmigration. Plates and kits are available in a wide range of membrane pore sizes to accommodate assays with adherent or suspension cell lines and to support cell growth for transmigration and coculture assays. The plates are provided sterile to permit longer incubation times and enable assay setup and analysis in the same device.
- Use with adherent or suspension cells
- Broad assay compatibility
- High assay consistency with little interassay variability
- Polycarbonate membrane available in 3, 5 and 8 μm pore sizes
- Pre-assembled kits available
MultiScreen® PCR filter plates
MultiScreen® PCR filter plates exploit size exclusion principles for purifying products of polymerase chain reaction (PCR), bacterial artificial chromosome (BAC) preparations, and plasmid preparations, as well sequencing reaction cleanup. These plates offer filters for fast, automatable, high-throughput PCR purification. Plates are provided in 96- and 384-well formats, including a micro-96-well format for purification of smaller volume PCR product samples.
- High purity and recovery
- Fast processing times
- >99.5% primer removal
- MultiScreen® PCR96 microwell filter plate recommended for small volumes (1 – 150 μL)
MultiScreen® vacuum manifolds and accessories
MultiScreen® vacuum manifolds use DirectStack™ technology to improve filter-based assay performance and reliability, enabling plate-on-plate stacking which eliminates gaps between flow directors and receiver wells in applications that require filtrate collection. A variety of accessories are available to optimize workflows for radioactivity, chromatography, and sample collection.
Looking for more information on Millicell® plates? Check out our extensive collection of Millicell® cell culture inserts and plates.
Related resources
- Article: A Highly Sensitive IFN-γ ELISpot Assay to Quantify Cellular Immune Responses to Previous Viral Infection
This study presents an example of a typical ELISpot assay for quantifying the number of pre-existing, antigen-reactive T cells from peripheral blood mononuclear cell (PBMC) samples obtained from donors previously infected with cytomegalovirus (CMV).
- Article: The ELISpot Assay Enables Functional Analysis of Cellular Immunology
ELISpot assay provides qualitative and quantitative information on immune responses, visualizing multiple secretory products from single responding cells.
- Flyer: MultiScreen® Plates and Applications Overview Chart
Access this handy, straightforward guide to determine the most appropriate plate types for specific application areas.
- Product Guide: Chemical Compatibility Guide for MultiScreen® Filter Plates
Check out this helpful reference guide for compatibility of MultiScreen®HTS and MultiScreen® Classic Filter Plates with various reagents.
- Flyer: MultiScreen®HTS Filter Plates for Automation
96-well and 384-well plates incorporate optimized glass fiber for assays including receptor-ligand binding.
- Flyer: MultiScreen®HTS Filter Plates for Automation
Advanced plate design for full automation compatibility applications.
- Flyer: MultiScreen®HTS Filter Plates for ELISpot
Optimized filter plates measure immune response on a single cell level.
- Application Note: Development of a Fluorescent ELISpot Duplexing Assay to Measure Immune Response in Cryopreserved PBMCs
Learn how a FluoroSpot assay was developed to evaluate cytokine production from CMV-seronegative and seropositive PBMCs at the single-cell level.
- Data Sheet: MultiScreen®-MIC Plates - 96-well system for cell-based functional screening assays including migration, invasion, and chemotaxis
Learn about the versatility, intra- and inter-lot reproducibility, and performance of MultiScreen®-MIC plates in angiogenesis and invasion assays.
- Application Note: Evaluation of MultiScreen®-MIC Plates in Chemotaxis Assays
Read about a study demonstrating the utility of MultiScreen®-MIC plates for chemotaxis assays using suspension and adherent cells.
- Data Sheet: MultiScreen®HTS Filter Plates - Advanced Plate Design for Full Automation Compatibility Applications
Learn about the features of MultiScreen®HTS plates for automation compatibility.
- Application Note: Evaluation of the reproducibility of Parallel Artificial Membrane Permeation Assays (PAMPA)
Learn how to optimize a drug permeability PAMPA assay for robust, reproducible data.
- Technical Article: ELISA versus ELISpot - Which Assay Should You Choose?
A comparison of key principles, applications, strengths, and limitations of ELISA and ELISpot.
Related videos
External resources
How to Choose Filter Plates for Automated Cell-based Assays (external link)
Learn why filter plates are used in cell-based assays and how to select a plate based on application, membrane, pore size, and automation needs.