Stability at Higher Temperatures

Purospher® STAR RP-18 endcapped
Mobile phase	Time	0.1 % v/v H3PO4 in water	Acetonitrile (%)
	0	95	5
	2	95	5
	12	80	80
	15	80	80
	15.1	95	5
	20	95	5
Column	Purospher® STAR RP-18 endcapped (3 µm) LiChroCART® 55-4
Flow rate	1.5 mL/min
Temperature	60°C
Sample	1. Amitriptyline 2. Valerophenone 3. o-Nitrophenol