Separation of Anisoles

Column	Purospher® STAR Si (5 µm) LiChroCART® 125-4
Eluent	n-Heptane/Dioxane (95/5, v/v)
Flow rate	2.0 mL/min
Detection	UV 254 nm fast response
Temperature	RT
Inj. volume	5 µL
Sample	1. Anisole 2. 3-Nitroanisole 3. 4-Nitroanisole 4. 2-Nitroanisole