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565759 β-Secretase Substrate V, Fluorogenic

β-Secretase Substrate V, Fluorogenic MSDS (material safety data sheet) or SDS, CoA and CoQ, dossiers, brochures and other available documents.

Synonyms: FS-2, MCA-SEVNLDAEFK(DNP)-CONH₂, Memapsin 2 Substrate II, Fluorogenic

565759
  
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      Overview

      Replacement Information
      Description
      OverviewA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate, based on the EVNLDAEF sequence derived from the β-secretase site of the Swedish mutation of Amyloid Precursor Protein (APP). Hydrolysis of the substrate (at the Leu-Asp bond) by β-secretase results in fluorescence enhancement. kcat/Km values (min-1/µM) at 37°C, pH 4.5, for pro-memapsin 2 and its activated species Leu28p-memapsin 2 and Gly45p-memapsin 2 are 0.056, 0.080 and 0.160, respectively.
      Catalogue Number565759
      Brand Family Calbiochem®
      SynonymsFS-2, MCA-SEVNLDAEFK(DNP)-CONH₂, Memapsin 2 Substrate II, Fluorogenic
      References
      ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.
      Product Information
      FormOff-white solid
      Hill FormulaC₆₈H₈₉N₁₅O₂₆
      Chemical formulaC₆₈H₈₉N₁₅O₂₆
      Applications
      Biological Information
      Purity≥95% by HPLC
      Physicochemical Information
      Emission max.
      Excitation max.
      Peptide SequenceMCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DNP)-CONH₂
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Ambient Temperature Only
      Toxicity Standard Handling
      Storage -20°C
      Protect from Light Protect from light
      Do not freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Packaging Information
      Packaged under inert gas Packaged under inert gas
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      565759 0

      Documentation

      References

      Reference overview
      Ermolieff, J., et al. 2000. Biochemistry 39, 12450.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision12-May-20-8 JSW
      SynonymsFS-2, MCA-SEVNLDAEFK(DNP)-CONH₂, Memapsin 2 Substrate II, Fluorogenic
      DescriptionA highly sensitive fluorescence resonance energy transfer (FRET) peptide substrate based on the GVNLDAEF sequence derived from the b-secretase site of the 'Swedish mutation' of Amyloid Precursor Protein (APP). Hydrolysis of the substrate at Leu-Asp bond by β-secretase results in a significant enhancement of fluorescence. Reported Kcat/Km values (min-1/µM) for pro-mempasin 2 and its proteolytically activated species Leu28p-mempasin 2 and Gly45p-mempasin 2 are 0.056, 0.080, and 0.160 respectively (0.1 M sodium acetate buffer, pH 4.5, 37°C). Excitation max.: ~328 nm; emission max.: ~393 nm.
      FormOff-white solid
      Intert gas (Yes/No) Packaged under inert gas
      Chemical formulaC₆₈H₈₉N₁₅O₂₆
      Peptide SequenceMCA-Ser-Glu-Val-Asn-Leu-Asp-Ala-Glu-Phe-Lys(DNP)-CONH₂
      Purity≥95% by HPLC
      SolubilityDMSO (5 mg/ml)
      Storage Protect from light
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 1 month at -20°C.
      Toxicity Standard Handling
      ReferencesErmolieff, J., et al. 2000. Biochemistry 39, 12450.