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AB3073 Anti-Aquaporin 6 Antibody, kidney specific form

AB3073
50 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB3073
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Aquaporin 6 Antibody, kidney specific form
      Alternate Names
      • AQP6
      • AQP2L
      Background InformationWater is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-6 (WCH3 or hKID or AQP2-like; 282 aa; 29 kDa; chromosome 12q13) is found only in the kidney with low water permeability. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. Aquaporin-6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%).
      References
      Product Information
      FormatAffinity Purified
      PresentationAffinity Purified immunoglobulin in PBS containing 0.1% BSA as stabilizer. No preservative.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Aquaporin 6 Antibody, kidney specific form is an antibody against Aquaporin 6 for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Application NotesWestern blot: 1-10 μg/mL using Chemiluminescence technique Immunohistochemistry: We recommend using the affinity purified antibody at 2-10 μg/mL in paraformaldehyde fixed sections of tissues.

      ELISA: 0.5-1.0 μg/mL

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenA 19 AA synthetic peptide within the carboxy terminal domain of human AQP6 (Ma et al. 1996) was selected for antibody production. This domain is predicted to be cytoplasmic.
      Epitopekidney specific form
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityWater is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special protein "aquaporin". Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. Aquaporin-0 or MIP26 (major intrinsic protein 26 kDa), and Aquaporin-1 (purified from red cells) also called CHIP-28 (channel forming integral protein, 28 kDa; 268 AA; gene locus 7p14) has been the foundation of the growing family of aquaporins. The lens specific Aquaporin-0 represents up to 80% of total lens membrane protein. Defects in MIP26 are a cause of autosomal dominant cataract. The cataract Fraser mutation (CAT-FR or Shriveled) is a transposon-induced splicing error that substitutes a long terminal repeat sequence for the c-terminus of MIP. The lens opacity mutation (LOP) is an AA substitution that inhibits targeting of MIP to the cell membrane. Aquaporin-6 (WCH3 or hKID or AQP2-like; 282 aa; 29 kDa; chromosome 12q13) is found only in the kidney with low water permeability. Aquaporin families of proteins are predicted to contain six transmembrane domains. The N and C-terminus are predicted to be cytoplasmic. Aquaporin-6 shows greatest homology with hMIP (48%) and hAQP-2 (52%). It also has similarity with human MIWC (AQP4; 34%), CHIP-28 (AQP1; 38%), and GLIP (AQP3; 22%)
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is an aquaporin protein, which functions as a water channel in cells. Aquaporins are a family of small integral membrane proteins related to the major intrinsic protein (MIP or AQP0). This protein is specific for the kidney. This gene and related family members AQP0, AQP2, and AQP5 reside in a cluster on chromosome 12q13.
      Gene Symbol
      • AQP6
      • hKID
      • AQP2L
      • KID
      • Aquaporin-6
      • AQP-6
      • Aquaporin-2-like
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q13520 # Forms a water-specific channel that participates in distinct physiological functions such as glomerular filtration, tubular endocytosis and acid-base metabolism (By similarity).
      SIZE: 282 amino acids; 29563 Da
      SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Multi-pass membrane protein (By similarity).
      DOMAIN:SwissProt: Q13520 Aquaporins contain two tandem repeats each containing three membrane-spanning domains and a pore-forming loop with the signature motif Asn-Pro-Ala (NPA).
      SIMILARITY: Belongs to the MIP/aquaporin (TC 1.A.8) family.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain frozen at -20°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size50 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Aquaporin 6 Antibody, kidney specific form SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Aquaporin 6 Antibody, kidney specific form Certificates of Analysis

      TitleLot Number
      RABBIT ANTI-AQUAPORIN-6 - 3190897 3190897
      RABBIT ANTI-AQUAPORIN-6 - 3267286 3267286
      RABBIT ANTI-AQUAPORIN-6 -2599286 2599286
      RABBIT ANTI-AQUAPORIN-6 AFFINITY PURIFIED POLYCLONAL ANTIBODY 2979061
      RABBIT ANTI-AQUAPORIN-6 AFFINITY PURIFIED POLYCLONAL ANTIBODY 3002793
      RABBIT ANTI-AQUAPORIN-6 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2197184 2197184
      RABBIT ANTI-AQUAPORIN-6 AFFINITY PURIFIED POLYCLONAL ANTIBODY - 2277865 2277865

      References

      Reference overviewApplicationPub Med ID
      Gene expression profiling of chromophobe renal cell carcinomas and renal oncocytomas by Affymetrix GeneChip using pooled and individual tumours.
      Yusenko, MV; Zubakov, D; Kovacs, G
      International journal of biological sciences  5  517-27  2009

      Show Abstract
      Western Blotting19680475 19680475
      Water permeability of cochlear outer hair cells: characterization and relationship to electromotility.
      Belyantseva, I A, et al.
      J. Neurosci., 20: 8996-9003 (2000)  2000

      Show Abstract
      11124975 11124975