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CBL553 Anti-CD63 Antibody, clone RFAC4

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100 µg  
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      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HFC, IP, WB, IHC, IH(P)MPurifiedMonoclonal Antibody
      Catalogue NumberCBL553
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-CD63 Antibody, clone RFAC4
      Product Information
      HS Code3002 15 90
      PresentationThe monoclonal is presented at a concentration of 100μg/1ml in phosphate buffered saline containing 10mM sodium azide and 1mg/ml bovine serum albumin. We recommend that each laboratory determine an optimum working titre for use in its particular application.
      Quality LevelMQ100
      ApplicationAnti-CD63 Antibody, clone RFAC4 is an antibody against CD63 for use in FC, IP, WB, IH, IH(P).
      Key Applications
      • Flow Cytometry
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry
      • Immunohistochemistry (Paraffin)
      Application NotesFlow cytometric studies of CD63 expression


      Western blotting of CD63 antigen from solubilized platelet proteins

      Suitable for formalin fixed paraffin wax embedded tissue sections. HIER and proteolytic pretreatment may not be necessary. Users should confirm specificity and pretreatment for their particular application.

      Optimal working dilutions must be determined by the end user.
      Biological Information
      SpecificityThis antibody reacts with a 53 kDa protein which form a part of the family of tetraspan moieties. The antigen was originally designated as a lysosomal membrane protein characterized as an activation dependent platelet surface antigen. In fact the CD63 antigen has a diverse distribution on the surface and in the cytoplasm of many cell types including lymphoid, myeloid, endothelial cells and melanoma.
      Species Reactivity
      • Human
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThe protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It may function as a blood platelet activation marker. Deficiency of this protein is associated with Hermansky-Pudlak syndrome. Also this gene has been associated with tumor progression. The use of alternate polyadenylation sites has been found for this gene. Alternative splicing results in multiple transcript variants encoding different proteins.
      Gene Symbol
      • CD63
      • TSPAN30
      • ME491
      • Tetraspanin-30
      • granulophysin
      • LAMP-3
      • tetraspanin-30
      • Granulophysin
      • MLA1
      • OMA81H
      • Lamp-3
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P08962 # This antigen is associated with early stages of melanoma tumor progression. May play a role in growth regulation.
      SIZE: 238 amino acids; 25637 Da
      SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein. Lysosome membrane; Multi-pass membrane protein. Late endosome membrane; Multi-pass membrane protein. Note=Also found in Weibel- Palade bodies of endothelial cells.
      TISSUE SPECIFICITY: Dysplastic nevi, radial growth phase primary melanomas, hematopoietic cells, tissue macrophages.
      SIMILARITY: SwissProt: P08962 ## Belongs to the tetraspanin (TM4SF) family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsFor use within 1 month of purchase store at +4°C, for long term storage aliquot antibody into small volumes and store at -20°C.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Global Trade Item Number
      Catalogue Number GTIN
      CBL553 04053252619212


      Anti-CD63 Antibody, clone RFAC4 SDS


      Safety Data Sheet (SDS) 

      Anti-CD63 Antibody, clone RFAC4 Certificates of Analysis

      TitleLot Number
      Anti-CD63, clone RFAC Monoclonal Anti 2846349
      Anti-CD63, clone RFAC4 2477003
      Anti-CD63, clone RFAC4 3027184
      Anti-CD63, clone RFAC4 - 2142169 2142169
      Anti-CD63, clone RFAC4 - 2391115 2391115
      Anti-CD63, clone RFAC4 - 2017681 2017681
      Anti-CD63, clone RFAC4 - 2042780 2042780
      Anti-CD63, clone RFAC4 - 2309060 2309060
      Anti-CD63, clone RFAC4 - 2554908 2554908
      Anti-CD63, clone RFAC4 - 3202401 3202401


      Reference overviewPub Med ID
      An acidic cluster of the cytoplasmic tail of the RD114 virus glycoprotein controls assembly of retroviral envelopes.
      David Bouard,Virginie Sandrin,Bertrand Boson,Didier Nègre,Gary Thomas,Christelle Granier,François-Loïc Cosset
      Traffic (Copenhagen, Denmark)  8  2007

      Show Abstract
      17547695 17547695