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CBL188 Anti-Clathrin Antibody, clone CHC5.9

100 µg  
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      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      Am, B, H, Po, RWB, IHCMPurifiedMonoclonal Antibody
      Catalogue NumberCBL188
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Clathrin Antibody, clone CHC5.9
      Product Information
      • Positive reactions using HeLa, SV-40 and RVF-SMC cultured cell lines.
      PresentationThe antibody was purified by gel filtration and is presented as 100μg of lyophilysed material. Reconstitute with 1ml distilled water. Final buffer composition is PBS, pH 7.4.
      Quality LevelMQ100
      ApplicationAnti-Clathrin Antibody, clone CHC5.9 is an antibody against Clathrin for use in WB, IH.
      Key Applications
      • Western Blotting
      • Immunohistochemistry
      Application NotesMarker for detection of receptor mediated endocytosis

      Suitable for frozen sections

      Western blotting KNOW SPECIES

      Optimal working dilutions must be determined by the end user.
      Biological Information
      ImmunogenCoated vesicles (clathrin) of bovine brain.
      SpecificityThis antibody reacts with the clathrin heavy chain from a diverse range of species. In nervous tissue it cross-reacts with another coated vesicle polypeptide of 190 kDa which is not present in isolated clathrin triskelions. In some epithelial cells of certain species the antibody may cross-react with a cytokeratin component. It detects coated vesicles from different organs and tissues (e.g. brain, ovary, mammary gland). : Recognizes bovine, rat and amphibian
      Species Reactivity
      • Amphibian
      • Bovine
      • Human
      • Pig
      • Rat
      Antibody TypeMonoclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryClathrin is a major protein component of the cytoplasmic face of intracellular organelles, called coated vesicles and coated pits. These specialized organelles are involved in the intracellular trafficking of receptors and endocytosis of a variety of macromolecules. The basic subunit of the clathrin coat is composed of three heavy chains and three light chains.
      Gene Symbol
      • CLTC
      • CHC17
      • CLTCL2
      • Hc
      • CLH17
      • CLH-17
      • KIAA0034
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: Q00610 # Clathrin is the major protein of the polyhedral coat of coated pits and vesicles. Two different adapter protein complexes link the clathrin lattice either to the plasma membrane or to the trans-Golgi network.
      SIZE: 1675 amino acids; 191615 Da
      SUBUNIT: Clathrin triskelions, composed of 3 heavy chains and 3 light chains, are the basic subunits of the clathrin coat. In the presence of light chains, hub assembly is influenced by both the pH and the concentration of calcium.
      SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane; Cytoplasmic side. Membrane, coated pit; Cytoplasmic side. Melanosome. Note=Cytoplasmic face of coated pits and vesicles. Identified by mass spectrometry in melanosome fractions from stage I to stage IV.
      SIMILARITY: SwissProt: Q00610 ## Belongs to the clathrin heavy chain family.
      Physicochemical Information
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain at 2-8°C for up to 12 months from date of receipt.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information


      Anti-Clathrin Antibody, clone CHC5.9 SDS


      Safety Data Sheet (SDS) 

      Anti-Clathrin Antibody, clone CHC5.9 Certificates of Analysis

      TitleLot Number
      MOUSE ANTI-CLATHRIN - 3524764 3524764
      MOUSE ANTI-CLATHRIN -2792012 2792012


      Reference overviewPub Med ID
      Association of adaptor protein TRIP8b with clathrin.
      Popova, NV; Deyev, IE; Petrenko, AG
      Journal of neurochemistry  118  988-98  2011

      Show Abstract
      21749376 21749376
      Molecular links between endocytosis and the actin cytoskeleton
      Qualmann, B, et al
      J Cell Biol, 150:F111-6 (2000)  2000

      10974009 10974009
      Clathrin and adaptors
      Hirst, J and Robinson, M S
      Biochim Biophys Acta, 1404:173-93 (1998)  1998

      9714795 9714795
      Identification of a distinct 9S form of soluble clathrin in cultured cells and tissues
      Bruder, G and Wiedenmann, B
      Exp Cell Res, 164:449-62 (1986)  1986

      3709679 3709679

      Data Sheet


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      Life Science Research > Antibodies and Assays > Primary Antibodies