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PC314 Anti-FAK (853-1052) Rabbit pAb

PC314
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~125 kDa as FAK protein in endothelial and NIH3T3 cells.
      Catalogue NumberPC314
      Brand Family Calbiochem®
      SynonymsAnti-FADK1, Anti-Focal Adhesion Kinase, Anti-p125FAK
      Application Data
      Detection of human FAK by immunoblotting. Sample: Human FAK. Primary antibody: Anti-FAK (853-1052) Rabbit pAb (Cat. No. PC314) (5 µg/ml). Detection: chemiluminescence.
      References
      ReferencesSchlaepfer, D.D., et al. 1998. Mol. Cell Biol. 18, 2571.
      Zachary, I. 1997. Int. J. Biochem. Cell Biol. 29, 929.
      Tachibana, K., et al. 1995. J. Exp. Med. 182, 1089.
      Schlaepfer, D.D., et al. 1994. Nature 372, 786.
      Hildebrand, J.D., et al. 1993. J. Cell Biol. 123, 993.
      Product Information
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Positive controlEndothelial or NIH 3T3 cells
      PreservativeNone
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Immunofluorescence
      Immunoprecipitation
      Application NotesImmunoblotting (5-10 µg/ml)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein)
      Application CommentsThis antibody has been reported to work for immunofluorescence studies localizing FAK in the cytoplasm and at focal contacts. Antibody should be titrated for optimal results in individual systems.
      Biological Information
      Immunogena recombinant protein consisting of amino acids 853-1052 of human FAK fused to GST
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-FAK (853-1052) Rabbit pAb Certificates of Analysis

      TitleLot Number
      PC314

      References

      Reference overview
      Schlaepfer, D.D., et al. 1998. Mol. Cell Biol. 18, 2571.
      Zachary, I. 1997. Int. J. Biochem. Cell Biol. 29, 929.
      Tachibana, K., et al. 1995. J. Exp. Med. 182, 1089.
      Schlaepfer, D.D., et al. 1994. Nature 372, 786.
      Hildebrand, J.D., et al. 1993. J. Cell Biol. 123, 993.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision03-Febuary-2009 RFH
      SynonymsAnti-FADK1, Anti-Focal Adhesion Kinase, Anti-p125FAK
      ApplicationImmunoblotting (5-10 µg/ml)
      Immunofluorescence (see comments)
      Immunoprecipitation (10 µl/mg protein)
      Application Data
      Detection of human FAK by immunoblotting. Sample: Human FAK. Primary antibody: Anti-FAK (853-1052) Rabbit pAb (Cat. No. PC314) (5 µg/ml). Detection: chemiluminescence.
      DescriptionPurified rabbit polyclonal antibody. Recognizes the ~125 kDa FAK protein.
      BackgroundFocal adhesion kinase (FAK) is a widely expressed non-receptor protein tyrosine kinase (PTK) that co-localizes with integrins in cellular focal adhesions by direct association with paxillin, a ~68 kDa cytoskeleton protein. FAK has been implicated in several cell processes including the regulation of cell motility, cell morphology and cell adhesion. Increased FAK tyrosine phosphorylation occurs upon integrin engagement with fibronectin and by a variety of extracellular factors including ligands for receptor tyrosine kinases and for seven transmembrane domain G-protein coupled receptors. FAK has a central catalytic domain and a C-terminal tail, a 159 amino acid C-terminal sequence (Focal adhesion targeting sequence) responsible for directing its cellular localization. Studies have shown that adhesion of murine NIH3T3 fibroblasts to fibronectin promotes association of the Grb2 adapter protein and c-Src PTK with FAK in vivo, and also results in activation of the ERK2 MAP kinase. In v-Src-transformed NIH3T3 cells, the association of v-Src, Grb2 and SOS with FAK is independent of cell adhesion to fibronectin. In vitro, the Grb2 SH2 domain binds directly to tyrosine phosphorylated FAK and the binding site has been identified as Tyr925 by site directed mutagenesis.
      HostRabbit
      Immunogen speciesHuman
      Immunogena recombinant protein consisting of amino acids 853-1052 of human FAK fused to GST
      IsotypeIgG
      Speciesamphibian, canine, human, mouse, rat
      Positive controlEndothelial or NIH 3T3 cells
      FormLiquid
      FormulationIn 10 mM PBS, pH 7.4.
      Concentration Label Please refer to vial label for lot-specific concentration
      PreservativeNone
      CommentsThis antibody has been reported to work for immunofluorescence studies localizing FAK in the cytoplasm and at focal contacts. Antibody should be titrated for optimal results in individual systems.
      Storage -20°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Standard Handling
      ReferencesSchlaepfer, D.D., et al. 1998. Mol. Cell Biol. 18, 2571.
      Zachary, I. 1997. Int. J. Biochem. Cell Biol. 29, 929.
      Tachibana, K., et al. 1995. J. Exp. Med. 182, 1089.
      Schlaepfer, D.D., et al. 1994. Nature 372, 786.
      Hildebrand, J.D., et al. 1993. J. Cell Biol. 123, 993.