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AB1954 Anti-Fibronectin Antibody

AB1954
100 µL  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      R, MELISA, WBRbAffinity PurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB1954
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-Fibronectin Antibody
      Alternate Names
      • Cold-insoluble globulin
      • fibronectin 1
      • migration stimulating factor
      • migration-stimulating factor
      Background InformationFibronectin (FN) is involved in many cellular processes, including tissue repair, embryogenesis, blood clotting, and cell migration/adhesion. Fibronectin exists in two main forms: 1) as an insoluble glycoprotein dimer that serves as a linker in the ECM (extracellular matrix), and; 2) as a soluble disulphide linked dimer found in the plasma (plasma FN). The plasma form is synthesized by hepatocytes, and the ECM form is made by fibroblasts, chondrocytes, endothelial cells, macrophages, as well as certain epithelial cells.
      References
      Product Information
      FormatAffinity Purified
      Control
      • Western Blot: Normal rat serum
      PresentationPurified in 0.1M Tris-Glycine (pH7.4) 150mM NaCl with 0.05% NaN3.
      Quality LevelMQ100
      Applications
      ApplicationAnti-Fibronectin Antibody detects level of Fibronectin & has been published & validated for use in ELISA & WB.
      Key Applications
      • ELISA
      • Western Blotting
      Biological Information
      ImmunogenPurified rat plasma fibronectin.
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityThe antibody reacts with the 262 kDa fibronectin protein.
      Species Reactivity
      • Rat
      • Mouse
      Species Reactivity NoteReacts with mouse and rat. Other species have not been tested.
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Entrez Gene SummaryThis gene encodes fibronectin, a glycoprotein present in a soluble dimeric form in plasma, and in a dimeric or multimeric form at the cell surface and in extracellular matrix. Fibronectin is involved in cell adhesion and migration processes including embryogenesis, wound healing, blood coagulation, host defense, and metastasis. The gene has three regions subject to alternative splicing, with the potential to produce 20 different transcript variants. However, the full-length nature of some variants has not been determined.
      Gene Symbol
      • FN1
      • DKFZp686O13149
      • DKFZp686H0342
      • DKFZp686I1370
      • MSF
      • FN
      • CIG
      • DKFZp686F10164
      • LETS
      • FINC
      Purification MethodAntigen Affinity Purified
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: P02751 # Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Interaction with TNR mediates inhibition of cell adhesion and neurite outgrowth (By similarity).
      SIZE: 2386 amino acids; 262607 Da
      SUBUNIT: Mostly heterodimers or multimers of alternatively spliced variants, connected by 2 disulfide bonds near the carboxyl ends; to a lesser extent homodimers. Interacts with FBLN1, AMBP, TNR, LGALS3BP and COL13A1.
      SUBCELLULAR LOCATION: Secreted, extracellular space, extracellular matrix.
      TISSUE SPECIFICITY: Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix. Ugl-Y1, Ugl-Y2 and Ugl-Y3 are found in urine.DEVELOPMENTAL STAGE: Ugl-Y1, Ugl-Y2 and Ugl-Y3 are present in the urine from 0 to 17 years of age.
      PTM: Sulfated.
      SIMILARITY: SwissProt: P02751 ## Contains 12 fibronectin type-I domains. & Contains 2 fibronectin type-II domains. & Contains 16 fibronectin type-III domains.
      Molecular Weight262 kDa
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssuranceRoutinely evaluated by Western Blot on normal rat serum.

      Western Blot Analysis:
      0.05 μg/mL - 1 μg/mL antibody detected fibronectin in 15 μL of normal rat serum.
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStable for 1 year at 2-8°C from date of receipt.
      Packaging Information
      Material Size100 µL
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-Fibronectin Antibody SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-Fibronectin Antibody Certificates of Analysis

      TitleLot Number
      Anti-Fibronectin (rabbit polyclonal) Polyclonal Antibody Q2925818
      Anti-Fibronectin - 2147066 2147066
      Anti-Fibronectin - 1944057 1944057
      Anti-Fibronectin - 1982481 1982481
      Anti-Fibronectin - 2043897 2043897
      Anti-Fibronectin - 2211027 2211027
      Anti-Fibronectin - 2325115 2325115
      Anti-Fibronectin - 3384872 3384872
      Anti-Fibronectin - 3466606 3466606
      Anti-Fibronectin - 3679449 3679449

      References

      Reference overviewApplicationSpeciesPub Med ID
      Protective effects of lipocalin-2 (LCN2) in acute liver injury suggest a novel function in liver homeostasis.
      Borkham-Kamphorst, E; van de Leur, E; Zimmermann, HW; Karlmark, KR; Tihaa, L; Haas, U; Tacke, F; Berger, T; Mak, TW; Weiskirchen, R
      Biochimica et biophysica acta  1832  660-73  2013

      Show Abstract
      Immunohistochemistry23376114 23376114
      CCN3/NOV small interfering RNA enhances fibrogenic gene expression in primary hepatic stellate cells and cirrhotic fat storing cell line CFSC.
      Borkham-Kamphorst, E; van Roeyen, CR; Van de Leur, E; Floege, J; Weiskirchen, R
      Journal of cell communication and signaling  6  11-25  2012

      Show Abstract
      21748432 21748432
      Chronic and intermittent hypoxia differentially regulate left ventricular inflammatory and extracellular matrix responses.
      Ramirez, TA; Jourdan-Le Saux, C; Joy, A; Zhang, J; Dai, Q; Mifflin, S; Lindsey, ML
      Hypertension research : official journal of the Japanese Society of Hypertension  35  811-8  2012

      Show Abstract
      Western BlottingRat22495609 22495609
      Cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.
      Welham, NV; Yamashita, M; Choi, SH; Ling, C
      PloS one  6  e17754  2011

      Show Abstract Full Text Article
      21423617 21423617
      Induction of lipocalin-2 expression in acute and chronic experimental liver injury moderated by pro-inflammatory cytokines interleukin-1β through nuclear factor-κB activation.
      Borkham-Kamphorst E, Drews F, Weiskirchen R
      Liver Int  31  656-65. doi  2011

      Show Abstract
      21457438 21457438
      SPARC mediates early extracellular matrix remodeling following myocardial infarction.
      McCurdy, SM; Dai, Q; Zhang, J; Zamilpa, R; Ramirez, TA; Dayah, T; Nguyen, N; Jin, YF; Bradshaw, AD; Lindsey, ML
      American journal of physiology. Heart and circulatory physiology  301  H497-505  2011

      Show Abstract
      21602472 21602472
      Mesenchymal stem cell responses to bone-mimetic electrospun matrices composed of polycaprolactone, collagen I and nanoparticulate hydroxyapatite.
      Phipps, MC; Clem, WC; Catledge, SA; Xu, Y; Hennessy, KM; Thomas, V; Jablonsky, MJ; Chowdhury, S; Stanishevsky, AV; Vohra, YK; Bellis, SL
      PloS one  6  e16813  2011

      Show Abstract Full Text Article
      21346817 21346817
      Correction: cross-sample validation provides enhanced proteome coverage in rat vocal fold mucosa.
      Welham NV, Yamashita M, Choi SH, Ling C
      PLoS One  6  2011

      Show Abstract
      21479201 21479201
      Vascular effects of cardiotrophin-1: a role in hypertension?
      Lopez-Andres, Natalia, et al.
      J. Hypertens., 28: 1261-72 (2010)  2010

      Show Abstract
      20216087 20216087
      In vivo matrix metalloproteinase-7 substrates identified in the left ventricle post-myocardial infarction using proteomics.
      Ying Ann Chiao,Rogelio Zamilpa,Elizabeth F Lopez,Qiuxia Dai,Gladys P Escobar,Kevin Hakala,Susan T Weintraub,Merry L Lindsey
      Journal of proteome research  9  2010

      Show Abstract Full Text Article
      20232908 20232908

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