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AB7824 Anti-p73 Antibody, α and β

AB7824
100 µg  
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      Overview

      Replacement Information

      Key Spec Table

      Species ReactivityKey ApplicationsHostFormatAntibody Type
      HIP, WB, IH(P)RbPurifiedPolyclonal Antibody
      Description
      Catalogue NumberAB7824
      Brand Family Chemicon®
      Trade Name
      • Chemicon
      DescriptionAnti-p73 Antibody, α and β
      Alternate Names
      • p53-like Transcription Factor
      References
      Product Information
      FormatPurified
      PresentationPurified immunoglobulin. 0.22 micron filtered. Provided at 1 mg/mL in PBS, pH 7.4, containing 0.05% sodium azide.
      Quality LevelMQ100
      Applications
      ApplicationAnti-p73 Antibody, α & β is a Rabbit Polyclonal Antibody for detection of p73 also known as p53-like Transcription Factor & has been validated in IP, WB, IHC(P).
      Key Applications
      • Immunoprecipitation
      • Western Blotting
      • Immunohistochemistry (Paraffin)
      Application NotesWestern blot: 1 μg/mL of the antibody will detect p73 in 30μg of total cell lysate from overexpressing cell lines. Higher levels of the antibody may be necessary when using native tissue sources.

      Immunoprecipitation: Add 1μg of antibody to a sample that has been precleared with Protein A Sepharose™ in 500 μl lysis buffer. Incubate antibody with sample at 4°C for 2 hours with agitation. Follow by incubation with 50 μL Protein A Sepharose™ for 2 hours at 4°C with agitation. Collect the Protein A complex by centrifugation, washing pellet 3 times in lysis buffer. Resuspend final sample in electrophesis buffer, denature by heating at 95°C for 5 minutes, and analyze by SDS-PAGE

      Immunohistochemistry of paraffin sections: Signal amplification may be necessary, in conjunction with standard IHC procedures. Dewax and rehydrate sections through descending graded alcohols to water using standard dewaxing protocols. Subject section to heat antigen retrieval. Wash sections 3 times with TBS, pH 7.4. Block endogenous peroxidase activity by incubating the section in 1% H2O2 in methanol for 30 minutes. Wash with TBS and detect according to preferred detection methods. With high sensitivity detection systems, typical working dilutions for the p73 antibody are 1:5,000 - 1;10,000.

      Optimal working dilutions must be determined by end user.
      Biological Information
      ImmunogenGST fusion protein containing aa450-636 of p73 protein.
      Epitopealpha and beta
      ConcentrationPlease refer to the Certificate of Analysis for the lot-specific concentration.
      HostRabbit
      SpecificityAntibody AB7824 recognizes amino acids 450-636 of p73 protein. This region is common to both p73alpha and p73beta isoforms, hence both isoforms of the p73 protein will be detected.
      Species Reactivity
      • Human
      Antibody TypePolyclonal Antibody
      Entrez Gene Number
      Gene Symbol
      • TP73
      • p73
      • P73
      UniProt Number
      UniProt SummaryFUNCTION: SwissProt: O15350 # Participates in the apoptotic response to DNA damage. When overproduced, activates transcription from p53-responsive promoters and induces apoptosis. May be a tumor suppressor protein.
      COFACTOR: Binds 1 zinc ion per subunit (By similarity).
      SIZE: 636 amino acids; 69623 Da
      SUBUNIT: The C-terminal oligomerization domain binds to the ABL1 tyrosine kinase SH3 domain. Isoform Beta interacts homotypically and with p53/TP53, whereas isoform Alpha does not. Isoform Gamma interacts homotypically and with all p73 isoforms. Isoform Delta interacts with isoform Gamma, isoform Alpha, and homotypically. Isoforms Alpha and Beta interact with HIPK2. Isoform Alpha interacts with RANBP9. Found in a complex with p53/TP53 and CABLES1. Isoform Beta interacts with WWOX. Interacts with HECW2.
      SUBCELLULAR LOCATION: Nucleus.
      TISSUE SPECIFICITY: Brain, kidney, placenta, colon, heart, liver, spleen, skeletal muscle, prostate, thymus and pancreas.
      DOMAIN: SwissProt: O15350 Possesses an acidic transactivation domain, a central DNA binding domain and a C-terminal oligomerization domain that binds to the ABL tyrosine kinase SH3 domain. & The WW-binding motif mediates interaction with WWOX.
      PTM: Isoform alpha (but not isoform beta) is sumoylated on Lys- 627, which potentiates proteasomal degradation but does not affect transcriptional activity.DISEASE:SwissProt: O15350 # Maps to a chromosome region frequently mutated in diverse cell lines of human cancer. Appears not to be frequently mutated in human cancers, in contrast to p53. Hemizygosity is observed in neuroblastoma and oligodendroglioma.
      SIMILARITY: Belongs to the p53 family.
      MISCELLANEOUS: Activated and stabilized by interaction with RANBP9.
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsMaintain refrigerated at 2-8°C in undiluted aliquots for up to 12 months.
      Packaging Information
      Material Size100 µg
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Anti-p73 Antibody, α and β SDS

      Title

      Safety Data Sheet (SDS) 

      Anti-p73 Antibody, α and β Certificates of Analysis

      TitleLot Number
      Anti p73 Antibody Rabbit polyclonal 2983171

      References

      Reference overviewPub Med ID
      Overexpression of phosphorylated-ATF2 and STAT3 in cutaneous angiosarcoma and pyogenic granuloma.
      Si-Yuan Chen,Satoshi Takeuchi,Kazunori Urabe,Sayaka Hayashida,Makiko Kido,Hiroto Tomoeda,Hiroshi Uchi,Teruki Dainichi,Masakazu Takahara,Satoko Shibata,Ya-Ting Tu,Masutaka Furue,Yoichi Moroi
      Journal of cutaneous pathology  35  2008

      Show Abstract
      18700251 18700251
      Differential expression of two new members of the p53 family, p63 and p73, in extramammary Paget's disease.
      S Chen, Y Moroi, K Urabe, S Takeuchi, M Kido, S Hayashida, H Uchi, T Uenotsuchi, Y Tu, M Furue
      Clinical and experimental dermatology  33  634-40  2008

      Show Abstract
      18627398 18627398
      Expression of p73 in normal skin and proliferative skin lesions.
      Makoto Kamiya, Yuko Takeuchi, Mari Katho, Hideaki Yokoo, Atsushi Sasaki, Yoichi Nakazato
      Pathology international  54  890-5  2004

      Show Abstract
      15598310 15598310
      Differential expression of p53 gene family members p63 and p73 in head and neck squamous tumorigenesis.
      Hong-Ran Choi, John G Batsakis, Feng Zhan, Erich Sturgis, Mario A Luna, Adel K El-Naggar
      Human pathology  33  158-64  2002

      Show Abstract
      11957139 11957139
      The expression of p73, p21 and MDM2 proteins in gliomas.
      Makoto Kamiya, Yoichi Nakazato, Makoto Kamiya, Yoichi Nakazato
      Journal of neuro-oncology  59  143-9  2002

      Show Abstract
      12241107 12241107
      Monoallelically expressed gene related to p53 at 1p36, a region frequently deleted in neuroblastoma and other human cancers.
      Kaghad, M, et al.
      Cell, 90: 809-19 (1997)  1997

      Show Abstract
      9288759 9288759
      p73 is a simian [correction of human] p53-related protein that can induce apoptosis.
      Jost, C A, et al.
      Nature, 389: 191-4 (1997)  1997

      Show Abstract
      9296498 9296498