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SCT212-60nmol HaloTag® AcidiFluor™ Orange Ligand

Live Cell imaging probe for acidic pH. It shows weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH.

SCT212-60nmol
60 nmol  
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      Overview

      Replacement Information
      Description
      Catalogue NumberSCT212-60nmol
      DescriptionHaloTag® AcidiFluor™ Orange Ligand
      OverviewHaloTag® AcidiFluorTM ORANGE is an acidic pH indicator. It consists of AcidiFluor ORANGE dye fused with HaloTag® ligand, which forms a covalent bond with Haotag. It shows quite weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH. Since it is cell impermeable reagent, it can be used to label membrane-associated HaloTag fused proteins just by mixing and then monitoring pH changes. Relatively photostable fluorescence of AcidiFluor™ ORANGE enables time lapse
      imaging of pH change and tracking of endocytic/exocytic vesicles for long-time period.

      Spectral Properties
      Absorbance: 520nm
      Emission: 565nm

      References
      Isa M et al. High-Throughput Screening System To Identify Small Molecules That Induce Internalization and Degradation of HER2. ACS Chem. Biol. 2014, 9, 10, 2237-2241.

      HaloTag® is a registered tradeMark of Promega Corporation.
      AcidiFluor™ is a trademark of Goryo Chemical Inc.
      Alternate Names
      • HaloTag® AcidiFluor™ ORANGE Ligand, 60 nmol
      References
      Product Information
      Detection methodFluorescence
      PresentationLyophilized
      Applications
      ApplicationLive Cell imaging probe for acidic pH. It shows weak fluorescence at neutral pH, but shows strong fluorescence at acidic pH.
      Key Applications
      • Cell Based Assays
      • Cell Surface Receptor Assays
      Application NotesLive cell fluorescent imaging. Orange fluorescence.
      Biological Information
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Quality AssurancePurity: ≥ 90% (LC)
      Usage Statement
      • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
      Storage and Shipping Information
      Storage ConditionsStore HaloTag® AcidiFluor™ Orange Ligand at -20°C, desiccate and protect from light
      Note: Centrifuge vial briefly to collect contents at bottom of vial before opening.
      Packaging Information
      Material Size60 nmol
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      SCT212-60nmol 0