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577814 PhosphoDetect™ Anti-Tau (pSer²⁶²) Rabbit pAb

577814
  
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      Overview

      Replacement Information

      Key Spec Table

      Host
      Rb
      Description
      Overview

      This product has been discontinued.





      Recognizes the ~45-68 kDa Tau protein phosphorylated at Ser262.
      Note: 1 T = 1 test.
      Catalogue Number577814
      Brand Family Calbiochem®
      Application Data
      Detection of human tau, phosphorylated at Ser262, by immunoblotting. Samples: Whole cell lysate from NIH3T3 cell with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer262) Rabbit pAb (Cat. No. 577814) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      References
      ReferencesBennecib, M., et al. 2001. FEBS Lett. 490, 15.
      del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
      Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
      Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
      Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
      Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
      Jenkins, S.M., and Johnson, G.V. 2000. J. Neurochem. 74, 1463.
      Maas, T., et al. 2000. J. Biol. Chem. 275, 15733.
      Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
      Schneider, A., et al. 1999. Biochemistry 38, 3549.
      Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
      Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
      Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
      Arigada, P.A., et al. 1992. Neurology 42, 631.
      Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.
      Product Information
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Positive controlHuman recombinant tau treated with GSK-3β or OV-1 cells stably expressing human four-repeat tau and SV40 small Tantigen.
      Preservative≤0.1% sodium azide
      Quality LevelMQ100
      Applications
      Key Applications Immunoblotting (Western Blotting)
      Application NotesImmunoblotting (1:1000)
      Application CommentsWhen used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. The immunogen sequence is 92% conserved in mouse and rat and 100% conserved in bovine, goat, baboon, and monkey tau. Variables associated with assay conditions will dictate the proper working dilution.
      Biological Information
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²⁶² phosphorylation site of human Tau
      ImmunogenHuman
      HostRabbit
      IsotypeIgG
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Blue Ice Only
      Toxicity Irritant
      Storage -20°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications
      Global Trade Item Number
      Catalogue Number GTIN
      577814 0

      Documentation

      PhosphoDetect™ Anti-Tau (pSer²⁶²) Rabbit pAb Certificates of Analysis

      TitleLot Number
      577814

      References

      Reference overview
      Bennecib, M., et al. 2001. FEBS Lett. 490, 15.
      del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
      Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
      Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
      Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
      Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
      Jenkins, S.M., and Johnson, G.V. 2000. J. Neurochem. 74, 1463.
      Maas, T., et al. 2000. J. Biol. Chem. 275, 15733.
      Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
      Schneider, A., et al. 1999. Biochemistry 38, 3549.
      Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
      Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
      Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
      Arigada, P.A., et al. 1992. Neurology 42, 631.
      Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.
      Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-August-2007 RFH
      ApplicationImmunoblotting (1:1000)
      Application Data
      Detection of human tau, phosphorylated at Ser262, by immunoblotting. Samples: Whole cell lysate from NIH3T3 cell with exogenously added human recombinant tau left untreated (lane 1) or treated with GSK-3β (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Tau (pSer262) Rabbit pAb (Cat. No. 577814) (1:1000) pre-incubated without peptide (lanes 1, 2), with non-phosphopeptide corresponding to the immunogen (lane 3), a generic phosphoserine containing peptide (lane 4), or phosphopeptide immunogen (lane 5). Detection: chemiluminescence.
      DescriptionRabbit polyclonal antibody adsorbed against non-phosphopeptide corresponding to the immunogen phosphorylation site, followed by immunoaffinity chromatography. Recognizes the ~45-68 kDa Tau protein phosphorylated at Ser262.
      BackgroundTau is the microtubule-associated protein found predominantly in neurons. It promotes the assembly of tubulin into microtubules. Tau is also a phosphoprotein and its biological activity is regulated by the degree of phosphorylation. In the brain of Alzheimer's patients, tau is hyperphosphorylated, and exists both as a cytosolic protein and as polymerized paired helical filaments (PHF). The PHF form the building blocks for the neurofibrillary tangles that are the hallmark of AD brains. Hyperphosphorylated tau in AD inhibits the assembly of microtubules and causes disassembly of preassembled microtubules. Ultimately, hyperphosphorylated tau leads to the degeneration of the neurons and forms the pathogenic mechanism for Alzheimer's disease. In addition to AD brains, neurofibrillary tangles have also been observed in brains of patients with Niemann-Pick type C (NPC) disease. NPC is associated with the accumulation of cholesterol in several tissues and with progressive neurodegeneration. It has recently been shown that brains of NPC patients also accumulate neurofibrillary tangles without amyloid deposits. As in AD brains, the PHF of NPC brains is the result of hyperphosphorylation of tau. Phosphorylation of tau at Thr231 by GSK-3 requires prior phosphorylation by protein kinase A at other sites. Phosphorylation at Thr231 and Ser262 is required for maximal inhibition of tau's binding to microtubules. CaM kinase II is the major kinase that phosphorylates Ser262 in the brain. Protein 14-3-3 ζ stimulates phosphorylation of Ser262 by protein kinase A.
      HostRabbit
      Immunogen speciesHuman
      Immunogena synthetic phosphopeptide corresponding to amino acids surrounding the Ser²⁶² phosphorylation site of human Tau
      IsotypeIgG
      Specieshuman, mouse, rat
      Positive controlHuman recombinant tau treated with GSK-3β or OV-1 cells stably expressing human four-repeat tau and SV40 small Tantigen.
      FormLiquid
      FormulationIn PBS, 1 mg/ml BSA, 50% glycerol, pH 7.3.
      Preservative≤0.1% sodium azide
      CommentsWhen used at a 1:1000 dilution, there is sufficient antibody for 10 immunoblots at 10 ml/blot. The immunogen sequence is 92% conserved in mouse and rat and 100% conserved in bovine, goat, baboon, and monkey tau. Variables associated with assay conditions will dictate the proper working dilution.
      Storage Avoid freeze/thaw
      -20°C
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
      Toxicity Irritant
      ReferencesBennecib, M., et al. 2001. FEBS Lett. 490, 15.
      del C. Alonso, A., et al. 2001. J. Biol. Chem. 276, 37967.
      Sawamura, N., et al. 2001. J. Biol. Chem. 276, 10314.
      Bennecib, M., et al. 2000. FEBS Lett. 485, 87.
      Hashiguchi, M., et al. 2000. J. Biol. Chem. 275, 25247.
      Jenkins, S.M., et al. 2000. Biochem. J. 345, 263.
      Jenkins, S.M., and Johnson, G.V. 2000. J. Neurochem. 74, 1463.
      Maas, T., et al. 2000. J. Biol. Chem. 275, 15733.
      Tesseur, I., et al. 2000. Am. J. Pathol. 156, 951.
      Schneider, A., et al. 1999. Biochemistry 38, 3549.
      Sengupta, A., et al. 1998. Arch. Biochem. Biophys. 357, 299.
      Wang, J.G., et al. 1998. FEBS Lett. 436, 28.
      Kopke, E., et al. 1993. J. Biol. Chem. 268, 24374.
      Arigada, P.A., et al. 1992. Neurology 42, 631.
      Drubin, D.G. and Kirschner, M.W. 1986. J. Cell. Biol. 103, 2739.

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