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662070 Ubiquitin-Activating Enzyme E1, Rabbit

662070
  
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      Overview

      Replacement Information

      Key Spec Table

      Description
      OverviewNative, rabbit ubiquitin-activating enzyme E1. Catalyzes the first step in the ubiquitin-protein isopeptide bond formation. Activates ubiquitin monomer at its C-terminal cysteine residue by forming a high energy thioester bond. This activated ubiquitin is then transferred to lysine residues on target proteins via the E2/E3 conjugation cascade. Critical component for the initiation of conjugation reactions in vitro.
      Catalogue Number662070
      Brand Family Calbiochem®
      References
      ReferencesTanaka, K. 1998. J. Biochem. (Tokyo) 123, 195.
      Ciechanover, A., et al. 1982. J. Biol. Chem. 257, 2537.
      Haas, A.L., and Rose, I.A. 1982. J. Biol. Chem. 257, 10329.
      Ciechanover, A., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 761.
      Product Information
      FormLiquid
      FormulationIn 50 mM HEPES, pH 8.0.
      Applications
      Biological Information
      Purity≥95% by SDS-PAGE
      Concentration Label Please refer to vial label for lot-specific concentration
      Physicochemical Information
      Dimensions
      Materials Information
      Toxicological Information
      Safety Information according to GHS
      Safety Information
      Product Usage Statements
      Storage and Shipping Information
      Ship Code Dry Ice Only
      Toxicity Standard Handling
      Storage ≤ -70°C
      Avoid freeze/thaw Avoid freeze/thaw
      Do not freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Packaging Information
      Transport Information
      Supplemental Information
      Specifications

      Documentation

      Ubiquitin-Activating Enzyme E1, Rabbit SDS

      Title

      Safety Data Sheet (SDS) 

      Ubiquitin-Activating Enzyme E1, Rabbit Certificates of Analysis

      TitleLot Number
      662070

      References

      Reference overview
      Tanaka, K. 1998. J. Biochem. (Tokyo) 123, 195.
      Ciechanover, A., et al. 1982. J. Biol. Chem. 257, 2537.
      Haas, A.L., and Rose, I.A. 1982. J. Biol. Chem. 257, 10329.
      Ciechanover, A., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 761.

      Brochure

      Title
      Caspases and other Apoptosis Related Tools Brochure
      Proteasomes Technical Bulletin

      Citations

      Title
    • Zhizhong Li, et al. (2006) Structure of a BMI-1-ring1B polycomb group ubiquitin ligase complex. Journal of Biological Chemistry 281, 20643-20649.
    • Data Sheet

      Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

      Revision16-September-2008 RFH
      DescriptionNative, rabbit ubiquitin-activating enzyme E1. Catalyzes the first step in ubiquitin-protein isopeptide bond formation. Activates ubiquitin monomer at its C-terminal cysteine residue by formatting a high energy thiolester bond. This activated ubiquitin is then transferred to lysine residues on target proteins via the E2/E3 conjugation cascade. Critical component for the initiation of conjugation reactions in vitro.
      FormLiquid
      FormulationIn 50 mM HEPES, pH 8.0.
      Concentration Label Please refer to vial label for lot-specific concentration
      Purity≥95% by SDS-PAGE
      Storage ≤ -70°C
      Avoid freeze/thaw
      Do Not Freeze Ok to freeze
      Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
      Toxicity Standard Handling
      ReferencesTanaka, K. 1998. J. Biochem. (Tokyo) 123, 195.
      Ciechanover, A., et al. 1982. J. Biol. Chem. 257, 2537.
      Haas, A.L., and Rose, I.A. 1982. J. Biol. Chem. 257, 10329.
      Ciechanover, A., et al. 1981. Proc. Natl. Acad. Sci. USA 78, 761.
      Citation
    • Zhizhong Li, et al. (2006) Structure of a BMI-1-ring1B polycomb group ubiquitin ligase complex. Journal of Biological Chemistry 281, 20643-20649.