Separation of Alkaloids


Column LiChrospher® 60 RP-select B (5 μm) LiChroCART® 250-4 

Merck:/Freestyle/LE-Lab-Essentials/Chromatography/Diagrams/LE-LiChrospherRP_select-B_Alkaloids-300x325-02042015.jpg
Mobile phase A: 0.05 mol/l KH2PO;
B: Methanol
Gradient 0 - 35 min 90% A - 55% A
35 - 40 min 55% A - 40% A
40 - 60 min 40% A
60 - 70 min 40% A - 25% A
70 - 80 min 25% A - 20% A
Flow rate 0.7 mL/min
Detection UV 285
Temperature Room temperature
Injection volume 20 μl
Sample

1. a-Homochelidonine
2. Chelidonine
3. Protopine
4. Allocryptopine
5. Stylopine
6. Coptisine
7. Berberine
8. Sanguinarine
9. Chelerythrine