On-Demand Webinar

Flow through Chromatography and Adsorptive Depth Filtration for Continuous Bioprocessing Applications

Duration: 1 Hour
  • Paul Beckett, Ph.D., Technology Consultant, Purification, Merck
  • Romas Skudas, Ph.D., Senior Scientist R&D, Purification, Merck
Abstract: Bind/elute chromatography steps are challenging to implement in continuous processes due to the step wise load/wash/elute/clean cycle used. Multiple columns are therefore utilised to provide a continuous elution stream but these are complex to install and control. Flow through methods are therefore far more conducive to continuous process operation.

Depth filters are overwhelmingly used in flow through modes in current bioprocesses already, purifying by a mixture of size exclusion and adsorptive mechanisms. The adsorptive capabilities of modern depth filter materials, with complex binding mechanisms to diatomaceous earth or activated carbon, are often underestimated and effective removal of soluble process contaminants can be achieved with this technology.

Flow through chromatography can be used for highly effective purification based on the novel implementation of combination technologies working in a continuous fashion. These combination technologies use orthogonal binding mechanisms to remove a broad spectrum of impurities.

Join us as we explore case studies on effective separation of host cell proteins (HCPs), antibody fragments and low molecular weight substances from solutions containing monoclonal antibodies. Monoclonal antibody recoveries of >85% and HCP reduction to <10ppm suggest that this platform can be adapted to standard and new purification templates whilst maintaining critical product quality attributes and achieving economically efficient biopharmaceutical molecule purification.

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