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407321 γ-Interferon, Rat, Recombinant, E. coli

407321
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Products

Numero di catalogoConfezionamento Qtà/conf
407321-100UG Fiala di plastica 100 μg
Description
OverviewRecombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
Catalogue Number407321
Brand Family Calbiochem®
References
ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
Product Information
FormLyophilized
FormulationLyophilized from 0.5X PBS, pH 7.2.
Quality LevelMQ100
Applications
Biological Information
Biological activityReported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
Purity≥95% by RP-HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Protect from Moisture Protect from moisture
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

γ-Interferon, Rat, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

γ-Interferon, Rat, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
407321

Riferimenti bibliografici

Panoramica delle referenze
Alvarado, J., et al. 2005. Immunol. Lett. 99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision22-July-2010 JSW
DescriptionRecombinant rat interferon-γ (IFN-γ) expressed in E. coli as a single, non-glycosylated polypeptide containing 135 amino acids. Purified by chromatographic methods. IFN-γ is a type II cytokine produced by activated T cells and natural killer cells. It plays a significant role immunoregulation and has been implicated in the induction of the expression of class II histocompatibility molecules in cells that do not normally express them, resulting in autoimmune disease.
FormLyophilized
FormulationLyophilized from 0.5X PBS, pH 7.2.
Purity≥95% by RP-HPLC
Biological activityReported as U/mg on a lot-specific basis using a cytopathic affect inhibition assay with NIH/3T3 cells challenged with EMC virus.
SolubilityReconstitute with sterile water to ≥100 µg/ml, containing 0.1% HSA or BSA. Further dilute with aqueous buffers just prior to use.
Storage -20°C
Protect from moisture
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, refrigerate (4°C) for short-term storage or aliquot and freeze (-20°C) for long-term storage. Stock solutions are stable for up to 7 days at 4°C or for up to 4 months at -20°C. Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling
ReferencesAlvarado, J., et al. 2005. Immunol. Lett. 99, 109.
Di Rosa, M., et al. 2005. Clin. Chem. Lab. Med. 43, 499.
Malaguarnera, L., et al. 2005. J. Clin. Lab. Anal. 19, 128.