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345806 β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli

345806
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
345806-50MIU Fiala di plastica 50 miu
Description
OverviewRecombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
Note: 1 mU = 1 milliunit.
Catalogue Number345806
Brand Family Calbiochem®
Synonymsβ1,4-D-Galactoside Galactohydrolase
References
ReferencesKierman, U.A., et al. 2004. Proteomincs 4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Product Information
CAS number9031-11-2
Activity≥1.5 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol <i>p</i>-nitrophenol from <i>p</i>-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
EC number3.2.1.23
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
Quality LevelMQ200
Applications
Biological Information
Specific Activity≥6 units/mg protein
Physicochemical Information
ContaminantsN-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage +2°C to +8°C
Do not freeze Yes
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

β1,4-Galactosidase, Streptococcus pneumoniae, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
345806

Riferimenti bibliografici

Panoramica delle referenze
Kierman, U.A., et al. 2004. Proteomincs 4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision30-June-2008 RFH
Synonymsβ1,4-D-Galactoside Galactohydrolase
DescriptionRecombinant, Streptococcus pneumoniae β1,4-Galactosidase expressed in E. coli. Catalyzes the hydrolysis of non-reducing terminal β1,4-linked galactose. This enzyme is not known to cleave substituted or branched galactose. Inhibited by fucose attached to penultimate sugar.
FormLiquid
FormulationIn 25 mM NaCl, 20 mM Tris-HCl, pH 7.5. Sterile solution.
Recommended reaction conditions50 mM sodium phosphate buffer, pH 6.0.
CAS number9031-11-2
EC number3.2.1.23
ContaminantsN-acetylglucosaminidase, α-galactosidase, α-mannosidase, neuraminidases, proteases: none detected. Recommended reaction buffer: 50 mM sodium phosphate buffer, pH 6.0
Specific activity≥6 units/mg protein
Activity≥1.5 units/ml
Unit definitionOne unit is defined as the amount of enzyme that will catalyze the release of 1.0 µmol p-nitrophenol from p-nitrophenyl-β-D-galactopyranoside per min at 37°C, pH 5.0.
Storage +2°C to +8°C
Do Not Freeze Yes
Toxicity Standard Handling
ReferencesKierman, U.A., et al. 2004. Proteomincs 4, 1825.
Prime, S., et al. 1996. J. Chromatogr. A. 720, 263.
Dwek, R.A., et al. 1993. Annu. Rev. Biochem. 62, 65.