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171272 AMPK Activator VI, RSVA314 - CAS 316130-82-2 - Calbiochem

171272
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Panoramica

Replacement Information

Tabella delle specifiche principali

CAS #Empirical Formula
316130-82-2C₁₈H₂₁N₃O₃

Products

Numero di catalogoConfezionamento Qtà/conf
171272-10MG Bottiglia di vetro 10 mg
Description
OverviewA cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC50 of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.
Catalogue Number171272
Brand Family Calbiochem®
SynonymsRSVA314, AMPK Signaling Activator VI
References
ReferencesVingtdeux, V., et al. 2011. FASEB J. 25, 219.
Product Information
CAS number316130-82-2
FormYellow solid
Hill FormulaC₁₈H₂₁N₃O₃
Chemical formulaC₁₈H₂₁N₃O₃
Structure formula ImageStructure formula Image
Quality LevelMQ100
Applications
Biological Information
Purity>90% by HPLC
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Shipped with Blue Ice or with Dry Ice
Toxicity Standard Handling
Storage -20°C
Protect from Light Protect from light
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Packaging Information
Packaged under inert gas Packaged under inert gas
Transport Information
Supplemental Information
Specifications

Documentation

AMPK Activator VI, RSVA314 - CAS 316130-82-2 - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

AMPK Activator VI, RSVA314 - CAS 316130-82-2 - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
171272

Riferimenti bibliografici

Panoramica delle referenze
Vingtdeux, V., et al. 2011. FASEB J. 25, 219.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision03-October-2011 RFH
SynonymsRSVA314, AMPK Signaling Activator VI
DescriptionA cell permeable hydrozide that activates AMPK indirectly via a CaMKKβ-dependent pathway similar to resveratrol (Cat. No. 554325), which results in the phosphorylation of Thr-172 on the AMPKα2 subunit. This activator promotes lysosomal amyloid-β degradation in 3 cell lines nearly 40 times more potently than resveratrol with an IC50 of 1-2 µM. Treatment with RSVA314 at 1-3 µM results in a dose-dependent inhibition of the phosphorylation of 3 proteins downstream from mTOR activation including p70S6K, eukaryotic elongation factor 2 kinase (eEF2K), and S6 ribosomal protein, and promotes a robust increase in the phosphorylation of the protein raptor at Ser-792 in western blots. At the same concentrations, it elicits a strong, dose-dependent conversion of the light chain 3 (LC3) protein, a marker involved with autophagy induction, from LC3-I and LC3-II, also in western blots.
FormYellow solid
Intert gas (Yes/No) Packaged under inert gas
CAS number316130-82-2
Chemical formulaC₁₈H₂₁N₃O₃
Structure formulaStructure formula
Purity>90% by HPLC
SolubilityDMSO (50 mg/ml)
Storage -20°C
Protect from light
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 3 months at -20°C.
Toxicity Standard Handling
ReferencesVingtdeux, V., et al. 2011. FASEB J. 25, 219.