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178611 Anti-Aquaporin 1 (243-261) Rabbit pAb

178611
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, R Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
178611-1ST Fiala di plastica 1 st
Description
OverviewRecognizes aquaporin-1 in red blood cells. Supplied with AQP1243-261 blocking peptide (immunogen).
Catalogue Number178611
Brand Family Calbiochem®
SynonymsAnti-AQP1, Anti-CHIP28
Application Data
Detection of rat aquaporin 1 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with the control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 1 (243-261) Rabbit pAb (Cat. No. 179611) (1:400). Detection: chemiluminescence.
References
ReferencesKing, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.
Product Information
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, pH 7.4 and 40 µg blocking peptide lyophilized from PBS.
Preservative≤0.1% sodium azide (antibody only)
Quality LevelMQ100
Applications
Key Applications Frozen Sections
Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1-2 µg/ml, chemiluminescence)
Frozen Sections (see comments)
Application CommentsThe epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 µg blocking peptide with 1 µg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
Biological Information
Immunogena synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1, conjugated to KLH
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDissolve the antibody in 50 µl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 µl deionized water and store in aliquots at -20°C.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Aquaporin 1 (243-261) Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
178611

Riferimenti bibliografici

Panoramica delle referenze
King, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-June-2007 RFH
SynonymsAnti-AQP1, Anti-CHIP28
ApplicationImmunoblotting (1-2 µg/ml, chemiluminescence)
Frozen Sections (see comments)
Application Data
Detection of rat aquaporin 1 by immunoblotting. Samples: Rat kidney membranes (lane 1) and preincubated with the control peptide antigen (lane 2). Primary antibody: Anti-Aquaporin 1 (243-261) Rabbit pAb (Cat. No. 179611) (1:400). Detection: chemiluminescence.
DescriptionProtein A purified rabbit polyclonal antibody. Recognizes the aquaporin-1 protein. Supplied with AQP1243-261 blocking peptide (immunogen).
BackgroundAQP1 is a 269 amino acid transmembrane protein belonging to the aquaporin family. AQP1 forms the major water transports pathway of red cells and renal proximal tubules and thin descending limb of Henle, but not collecting duct.
HostRabbit
Immunogen speciesHuman
Immunogena synthetic peptide (KVWTSGQVEEYDLDADDIN) corresponding to amino acids 243-261 of human AQP1, conjugated to KLH
IsotypeIgG
Specieshuman, rat
FormLyophilized
Formulation50 µl antibody lyophilized from PBS, pH 7.4 and 40 µg blocking peptide lyophilized from PBS.
Preservative≤0.1% sodium azide (antibody only)
CommentsThe epitope is unique for AQP1 and is not present in other known related proteins. It is identical in human, rodent, and cattle, and only slightly different in frog. Recognition of AQP1 can be fully inhibited with the soluble AQP1243-261 blocking peptide. For use as a negative control, preincubate 1 µg blocking peptide with 1 µg antibody for 1 h at room temperature. This antibody is also reported to work with frozen sections. Antibody should be titrated for optimal results in individual systems.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDissolve the antibody in 50 µl deionized water to make a 1 mg/ml stock solution. Further dilutions should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Avoid freeze/thaw cycles of stock solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10000 x g for 5 min) to remove any aggregates. Dissolve the peptide in 100 µl deionized water and store in aliquots at -20°C.
Toxicity Standard Handling
ReferencesKing, L.S., et al. 1997. Am. J. Physiol. 73, C1541.
Nielson, S., et al. 1997. Am. J. Physiol. 273, C154.
Preston, G.M., and Agre, P. 1991. Proc. Natl. Acad. Sci. USA 88, 11110.