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681507 Anti-CaV1.2 (818-835) Rabbit pAb

681507
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
M, Rb, R Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
681507-1ST Fiala di plastica 1 st
Description
OverviewRecognizes the ~190 kDa and ~210 forms of the α1C-subunit. Supplied with a control peptide. Note: The α-subunit is highly sensitive to proteases. Please refer to the data sheet for proper sample preparation.
Catalogue Number681507
Brand Family Calbiochem®
SynonymsAnti-L-Type Ca2+ Channel
Application Data
Detection of rat CaV1.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:200). Detection: chemiluminescence.

Detection of mouse CaV1.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV1.2 channel (red) appearing in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer (Figure B). Confocal merge of CaV1.2 and CBD28K (Figure C). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:100). Detection: fluorescence.
References
ReferencesHell, J.W., et al. 1995. EMBO J. 14, 3036.
Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Snutch, T.P. et al. 1991. Neuron 7, 45.
Product Information
FormLyophilized
Formulation50 µl antibody in PBS, 5% sucrose, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
Positive controlRat brain or heart
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunocytochemistry Hell, J.W., et al. 1995. EMBO J. 14, 3036. Immunoprecipitation Hell, J.W., et al. 1995. EMBO J. 14, 3036. Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Key Applications Immunoprecipitation
Immunoblotting (Western Blotting)
Immunocytochemistry
Immunohistochemistry
Application NotesImmunoblotting (1:200)
Immunocytochemistry (see application references)
Immunoprecipitation (see application references)
Immunohistochemistry (!:100)
Application CommentsFor use as a negative control, preincubate 1 µg of CaV1.2 control peptide with 1 µg of antibody for 1 h at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide [(C)TTKINMDDLQPSENEDKS] corresponding to amino acids 848-865 of α1C-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
ImmunogenRat
HostRabbit
IsotypeIgG
Species Reactivity
  • Mouse
  • Rabbit
  • Rat
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsDilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the CaV1.2 control peptide with 100 µl deionized H₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of antibody and peptide solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10,000 x g for 5 min) to remove any aggregates.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-CaV1.2 (818-835) Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
681507

Riferimenti bibliografici

Panoramica delle referenze
Hell, J.W., et al. 1995. EMBO J. 14, 3036.
Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Snutch, T.P. et al. 1991. Neuron 7, 45.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision15-August-2011 JSW
SynonymsAnti-L-Type Ca2+ Channel
ApplicationImmunoblotting (1:200)
Immunocytochemistry (see application references)
Immunoprecipitation (see application references)
Immunohistochemistry (!:100)
Application Data
Detection of rat CaV1.2 by immunoblotting. Samples: Extract from rat brain membranes left untreated (lane 1) and treated by preincubation with the peptide antigen (lane 2). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:200). Detection: chemiluminescence.

Detection of mouse CaV1.2 by immunohistochemistry. Samples: Mouse cerebellum with CaV1.2 channel (red) appearing in Purkinje cells (horizontal arrows) and is distributed diffusely in the molecular layer (Mol) including in Purkinje dendrites (vertical arrows) (Figure A). Staining of Purkinje nerve cells with mouse anti-calcium binding protein antibody (green) in the section demonstrates the location of dendrites in the molecular layer (Figure B). Confocal merge of CaV1.2 and CBD28K (Figure C). Primary antibody: Anti-CaV1.2 (818-835) Rabbit pAb (Cat. No. 681507) (1:100). Detection: fluorescence.
DescriptionImmunoaffinity purified Rabbit polyclonal antibody. Recognizes the ~190 kDa and the ~210 kDa forms of the α1C-subunit of the voltage-gated Ca2+ channel. Supplied with a control peptide (immunogen).
HostRabbit
Immunogen speciesRat
Immunogena synthetic peptide [(C)TTKINMDDLQPSENEDKS] corresponding to amino acids 848-865 of α1C-subunit of rat brain voltage-gated calcium channels, conjugated to KLH
IsotypeIgG
Speciesmouse, rabbit, rat
Positive controlRat brain or heart
FormLyophilized
Formulation50 µl antibody in PBS, 5% sucrose, 1% BSA, pH 7.4 and 40 µg lyophilized control peptide (immunogen).
PreservativeNone
CommentsFor use as a negative control, preincubate 1 µg of CaV1.2 control peptide with 1 µg of antibody for 1 h at room temperature. Caution: α1-subunits of voltage-gated Ca2+ channels are highly sensitive to proteases. All procedures involving a full-length protein should be performed at 4°C and include the following protease inhibitor cocktail: pepstatin A (Cat. No. 516482; 1 µg/ml), leupeptin (Cat. No. 108975; 1 µg/ml), aprotinin (Cat. No. 616398; 1 µg/ml), benzamidine (Cat. No. 199001; 0.1 µg/ml), AEBSF (Cat. No. 101500; 0.2 mM) and calpain inhibitors I (LLNL, Cat. No. 208719; 8 µg/ml) and II (ALLM, Cat. No. 208721; 8 µg/ml). Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsDilutions of the antibody should be made in a physiological buffer containing a carrier protein such as 1-3% BSA. Reconstitute the CaV1.2 control peptide with 100 µl deionized H₂O, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of antibody and peptide solutions. To reduce nonspecific staining, centrifuge all antibody preparations before use (10,000 x g for 5 min) to remove any aggregates.
Toxicity Standard Handling
ReferencesHell, J.W., et al. 1995. EMBO J. 14, 3036.
Westenbroek, R.E., et al. 1995. J. Neurosci. 15, 6403.
Hell, J.W., et al. 1993. J. Cell Biol. 123, 949.
Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.
Snutch, T.P. et al. 1991. Neuron 7, 45.
Application referencesImmunocytochemistry Hell, J.W., et al. 1995. EMBO J. 14, 3036. Immunoprecipitation Hell, J.W., et al. 1995. EMBO J. 14, 3036. Dubel, S.J., et al. 1992. Proc. Natl. Acad. Sci. USA 89, 5058.