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219010 Anti-Catalase Rabbit pAb

219010
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
219010-1ML Fiala di plastica 1 ml
Description
OverviewRecognizes the ~60-65 kDa catalase protein.
Catalogue Number219010
Brand Family Calbiochem®
Application Data
Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
References
Product Information
FormLiquid
FormulationIn PBS.
Positive controlRed blood cells
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Application ReferencesImmunoblotting, Paraffin Sections Meilhac, O., et al. 2001. Arterioscler. Thromb. Vasc. Biol. 21, 1681.
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoelectrophoresis
Application NotesIndirect ELISA (1:128)
Immunoblotting (1:8000; see application references)
Immunoelectrophoresis (see comments)
Paraffin Sections (see application references)
Application CommentsThe 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenpurified, human erythrocytes catalase
ImmunogenHuman
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Catalase Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Catalase Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
219010

Citazioni

Titolo
  • Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry 282, 26245-26256.
  • Scheda tecnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision25-July-2007 RFH
    ApplicationIndirect ELISA (1:128)
    Immunoblotting (1:8000; see application references)
    Immunoelectrophoresis (see comments)
    Paraffin Sections (see application references)
    Application Data
    Detection of human catalase by immunoblotting. Samples: Low molecular weight markers (lanes 1, 5a, 5b, 9), catalase protein (0.075 µg) (lanes 2 and 6), catalase protein (0.15 µg) (lanes 3 and 7), and catalase protein (0.3 µg) (lanes 4 and 8), and red blood cell lysate (lane 10). With primary antibody dilution 1:8000 (lanes 1-5a) and 1:6000 (lanes 5b-10). Primary antibody: Anti-Catalase Rabbit pAb (Cat. No. 219010) (1:6000). Detection: chemiluminescence.
    DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and DEAE-cellulose ion exchange chromatography. Recognizes the ~60-65 kDa (monomer MW) catalase protein under reducing conditions.
    HostRabbit
    Immunogen speciesHuman
    Immunogenpurified, human erythrocytes catalase
    IsotypeIgG
    Specieshuman, mouse
    Positive controlRed blood cells
    FormLiquid
    FormulationIn PBS.
    Concentration Label Please refer to vial label for lot-specific concentration
    Preservative≤0.1% sodium azide
    CommentsThe 256 kDa catalase protein is a tetramer of 4 identical ~60-65 kDa protein. Exhibits a single arc by immunoelectrophoresis (IEP) vs. human red cell lysate. Variables associated with assay conditions will dictate the proper working dilution.
    Storage -20°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
    Toxicity Standard Handling
    Citation
  • Trine Nilsen, et al. (2007) A nuclear export sequence located on a β-strand in fibroblast growth factor-1. Journal of Biological Chemistry 282, 26245-26256.
  • Application referencesImmunoblotting, Paraffin Sections Meilhac, O., et al. 2001. Arterioscler. Thromb. Vasc. Biol. 21, 1681.