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219350 Anti-β-Catenin, Dephospho Mouse mAb (8E4)

219350
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M, R M Monoclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
219350-1SET Fiala di plastica 1 set
Description
OverviewRecognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
Catalogue Number219350
Brand Family Calbiochem®
Application Data
Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
References
Product Information
FormLyophilized
Formulation100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
Positive controlLiCl-treated SW480 cells (included)
Quality LevelMQ100
Applications
Key Applications Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunoprecipitation
Application NotesELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
Application CommentsSW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
ImmunogenMouse
Clone8E4
HostMouse
IsotypeIgG₁
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage -20°C
Do not freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-β-Catenin, Dephospho Mouse mAb (8E4) MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-β-Catenin, Dephospho Mouse mAb (8E4) Certificati d'Analisi

TitoloNumero di lotto
219350
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision25-July-2007 RFH
ApplicationELISA (0.05 µg/ml)
Immunoblotting (1 µg/ml colorimetric, 0.1 µg/ml, chemiluminescence)
Immunoprecipitation (1-10 µg/10⁶ LiCl-treated SW480 cells)
Application Data
Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
DescriptionMouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
Backgroundβ-Catenin is a cytoplasmic protein that mediates the interaction of Ca2+-dependent transmembrane adhesion proteins (cadherins) with the cytoskeletal network. β-Catenin may also play a role in tumorigenesis, as it complexes with the tumor suppressor gene product APC. β-Catenin directly interacts and constitutively activates transcription factors of the TCF/LEF family.
HostMouse
Immunogen speciesMouse
Immunogena synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
Clone8E4
IsotypeIgG₁
Specieshuman, mouse, rat
Positive controlLiCl-treated SW480 cells (included)
FormLyophilized
Formulation100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
CommentsSW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Do Not Freeze Ok to freeze
Special InstructionsReconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Toxicity Standard Handling