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219350
Sigma-AldrichAnti-β-Catenin, Dephospho Mouse mAb (8E4)
This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho.
More>>This Anti-β-Catenin, Dephospho Mouse mAb (8E4) is validated for use in ELISA, Immunoblotting, Immunoprecipitation for the detection of β-Catenin, Dephospho. Less<<
Anti-β-Catenin, Dephospho Mouse mAb (8E4): MSDS (material safety data sheet) o SDS, certificato d’analisi (CoA) e certificato di qualità (CoQ), dossier, brochure e altri documenti disponibili.
Recognizes the ~90 kDa nuclear dephospho-β-catenin in vanadate treated A431 cells. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
Catalogue Number
219350
Brand Family
Calbiochem®
Application Data
Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
References
Product Information
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
SW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogen
a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
Immunogen
Mouse
Clone
8E4
Host
Mouse
Isotype
IgG₁
Species Reactivity
Human
Mouse
Rat
Antibody Type
Monoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code
Ambient Temperature Only
Toxicity
Standard Handling
Storage
-20°C
Do not freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
Anti-β-Catenin, Dephospho Mouse mAb (8E4) Certificati d'Analisi
Titolo
Numero di lotto
219350
Scheda tecnica
Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.
Detection of human dephospho-β-catenin by immunoblotting. Samples: Whole cell lysates of serum starved A431 cells (lane 1), SW480 cells (lane 2), SW620 cells (lane 3), HT29 cells (lane 4), MCF7 cells (lane 5), MDA-MB231 cells (lane 6), and T47D cells (lane 7). Primary antibody: Anti-β-Catenin, Dephospho Mouse mAb (8E4) (Cat. No. 219350) (0.5 µg/ml). Detection: chemiluminescence.
Description
Mouse monoclonal antibody purified by thiophilic adsorption and size exclusion chromatography. Recognizes the ~90 kDa nuclear dephospho β-catenin protein. Supplied with a control lysate derived from 2x106 LiCl-treated SW480 cells.
Background
β-Catenin is a cytoplasmic protein that mediates the interaction of Ca2+-dependent transmembrane adhesion proteins (cadherins) with the cytoskeletal network. β-Catenin may also play a role in tumorigenesis, as it complexes with the tumor suppressor gene product APC. β-Catenin directly interacts and constitutively activates transcription factors of the TCF/LEF family.
Host
Mouse
Immunogen species
Mouse
Immunogen
a synthetic peptide corresponding to amino acids 27-37 of mouse β-catenin, conjugated to KLH
Clone
8E4
Isotype
IgG₁
Species
human, mouse, rat
Positive control
LiCl-treated SW480 cells (included)
Form
Lyophilized
Formulation
100 µg antibody lyophilized from 2X PBS, sucrose, PEG and 200 µl lyophilized control lysate.
Comments
SW480 LiCl cell lysate is recommended for immunoblot applications at a concentration of 20 µl/lane (mini gel) for chemiluminescent detection. Variables associated with assay conditions will dictate the proper working dilution.
Storage
-20°C
Do Not Freeze
Ok to freeze
Special Instructions
Reconstitute antibody with 1 ml dH₂O. Following reconstitution, refrigerate (4°C) for short-term storage or aliqout, flash-freeze in liquid nitrogen, and deep freeze (-70°C) for long-term storage. Stock solutions are stable for up to 3 months at 4°C or for up to 1 year at -70°C. Thaw aliquots at 37°C. Reconstitute positive control lysate with 200 µl H₂O. After complete solubilization of the proteins add 200 µl SDS-PAGE sample buffer and incubate at 90°C for 5 min. Following reconstitution of positive control, aliquot and freeze (-20°C). Avoid freeze/thaw cycles of solutions.
