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400064 Anti-Glucose Transporter-4 Rabbit pAb

400064
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
H, M, R Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
400064-50UG Fiala di plastica 50 μg
Description
OverviewRecognizes the ~40-43 kDa GLUT-4 protein.
Catalogue Number400064
Brand Family Calbiochem®
SynonymsAnti-Glut-4
References
ReferencesMueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
Product Information
FormLiquid
FormulationIn PBS, 0.1% BSA, pH 7.5.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoprecipitation
Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesELISA (0.5-1 µg/ml)
Frozen Sections (2-20 µg/ml)
Immunoblotting (1-10 µg/ml; see comments)
Immunoprecipitation (1-10 µg/100 µg tissue membrane)
Application CommentsDoes not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
Immunogena synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
ImmunogenMouse
HostRabbit
IsotypeIgG
Species Reactivity
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Glucose Transporter-4 Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Glucose Transporter-4 Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
400064

Riferimenti bibliografici

Panoramica delle referenze
Mueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision13-March-2009 RFH
SynonymsAnti-Glut-4
ApplicationELISA (0.5-1 µg/ml)
Frozen Sections (2-20 µg/ml)
Immunoblotting (1-10 µg/ml; see comments)
Immunoprecipitation (1-10 µg/100 µg tissue membrane)
DescriptionImmunoaffinity purified rabbit polyclonal antibody. Recognizes the ~40-43 kDa Glut-4 protein.
BackgroundGlucose transporter-4 (Glut-4) is a transmembrane protein that facilitates transport of glucose in the cell in an energy-independent fashion. Insulin activates glucose transport in fat cells through a translocation of the insulin-sensitive Glut-4 and by activation of glucose carriers in the plasma membrane. Glut-4 is unique to tissues that exhibit insulin-stimulated glucose uptake (heart and skeletal muscle and adipose tissue).
HostRabbit
Immunogen speciesMouse
Immunogena synthetic peptide corresponding to amino acids near the C-terminus of mouse GLUT-4, conjugated to KLH
IsotypeIgG
Specieshuman, mouse, rat
FormLiquid
FormulationIn PBS, 0.1% BSA, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsDoes not cross-react with Hep G2-type transporter or other Glut isoforms. Important: Do not heat samples prior to immunoblotting, as heating will result in protein aggregation. Variables associated with assay conditions will dictate the optimal working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesMueckler, M. 1994. Eur. J. Biochem. 219, 713.
James, D.E., et al. 1989. Nature 338, 83.
James, D.E., et al. 1989. Proc. Natl. Acad. Sci. USA 86, 8368.