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393207 Anti-HNE-Michael Adducts, Reduced Rabbit pAb

393207
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
A Broad Range Of Species Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
393207-100UL Fiala di plastica 100 ul
Description
OverviewRecognizes chemically reduced amino acid-(4)-HNE adducts. Does not recognize non-reduced adducts or reduced modifications arising from malondialdehyde (MDA) and structurally-related alkenes such as trans-2-noneal.
Catalogue Number393207
Brand Family Calbiochem®
SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
References
ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
Uchida, K., et al. 1994. Biochemistry 33, 12487.
Product Information
FormLiquid
FormulationSerum diluted in 50% glycerol, pH 7.5.
PreservativeNone
Quality LevelMQ100
Applications
Key Applications Paraffin Sections
Enzyme-Linked Immunosorbent Assay
Frozen Sections
Immunoblotting (Western Blotting)
Application NotesELISA (1:500-1:1000)
Immunoblotting (1:1000-1:4000)
Frozen Sections (1:50-1:500)
Paraffin Sections (1:50-1:500)
Application CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Biological Information
ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
HostRabbit
IsotypeIgG
Species Reactivity
  • A Broad Range Of Species
Antibody TypePolyclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-HNE-Michael Adducts, Reduced Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-HNE-Michael Adducts, Reduced Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
393207

Riferimenti bibliografici

Panoramica delle referenze
Toyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
Uchida, K., et al. 1994. Biochemistry 33, 12487.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision02-August-2007 RFH
SynonymsAnti-4-Hydroxy-2-noneal Michael Adducts, Reduced
ApplicationELISA (1:500-1:1000)
Immunoblotting (1:1000-1:4000)
Frozen Sections (1:50-1:500)
Paraffin Sections (1:50-1:500)
DescriptionRabbit polyclonal antibody supplied as diluted serum. Recognizes, specifically, chemically reduced amino acid-(4)-HNE adducts.
BackgroundThe CNS is enriched in polyunsaturated fatty acids and is therefore particularly vulnerable to lipid peroxidation. 4-Hydroxy-2-noneal (HNE) is one of the major lipid peroxidation products with cytotoxic and mutagenic activity. It reacts with protein residues such as histidine to generate stable Michael adducts. HNE has been shown to inhibit neurite outgrowth and disrupt microtubules and tubulin modification, suggesting it may play a role in Alzheimer's disease.
HostRabbit
ImmunogenHNE-treated KLH in which 1:1 amino acid-HNE Michael adducts were chemically reduced
IsotypeIgG
Speciesa broad range of species
FormLiquid
FormulationSerum diluted in 50% glycerol, pH 7.5.
PreservativeNone
CommentsDoes not bind non-reduced adducts and reduced modifications arising from malondialdehyde (MDA) or structurally-related alkenals such as trans-2-noneal. Does not cross-react with non-reduced amino acid-HNE Michael adducts. Antibody binding requires the presence of the 4-hydroxyl group. Variables associated with assay conditions will dictate the optimal working dilution.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
ReferencesToyokuni, S., et al. 2000. Antioxid. Redox. Signal 2, 681.
Neely, M.D., et al. 1999. J. Neurochem. 72, 2323.
Uchida, K., et al. 1995. Biochem. Biophys. Res. Commun. 212, 1068.
Uchida, K., et al. 1995. Arch. Biochem. Biophys. 324, 241.
Uchida, K., et al. 1994. Biochemistry 33, 12487.