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407143 Anti-IP₃ Receptor Rabbit pAb

407143
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
B, Ca, H, M, Porcine, R Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
407143-100UG Fiala di plastica 100 μg
Description
OverviewRecognizes all of the ~260 kDa IP3 receptor subtypes.
Catalogue Number407143
Brand Family Calbiochem®
References
ReferencesBourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
Product Information
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunofluorescence
Immunohistochemistry
Immunoprecipitation
Radioimmunoassay
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Immunoblotting (Western Blotting)
Application NotesELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:250)
Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Radioimmunoassay (1:10000)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.

Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies

NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.

• TBST
• 20 mM Tris-HCl, pH 7.6
• 150 mM NaCl
• 0.05% Tween®-20 detergent

1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
6. Shake for 1 h.
7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
8. Mix development solution (1:1) (Amersham ECL kit).
9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Biological Information
Immunogena synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Canine
  • Human
  • Mouse
  • Porcine
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Blue Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-IP₃ Receptor Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-IP₃ Receptor Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
407143

Riferimenti bibliografici

Panoramica delle referenze
Bourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
ApplicationELISA (1:2000)
Flow Cytometry (1:1000)
Immunoblotting (1:250)
Immunofluorescence (1:200)
Immunohistochemistry (1:200)
Immunoprecipitation (1:200)
Radioimmunoassay (1:10000)
DescriptionRabbit polyclonal antibody purified by ammonium sulfate precipitation and ion-exchange (DEAE) column chromatography. Recognizes the ~260 kDa IP3 receptor protein.
BackgroundInositol 1,4,5-triphosphate (IP3) receptors have been shown to play an important role in the regulation of Ca2+ release from internal storage sites during cell activation.
HostRabbit
Immunogena synthetic peptide corresponding to amino acids in the C-terminal cytoplasmic domain of the IP₃ receptor
IsotypeIgG
Speciesbovine, canine, human, mouse, porcine, rat
FormLiquid
FormulationIn 150 mM NaCl, 10 mM sodium phosphate, pH 7.5.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsVariables associated with assay conditions will dictate the proper working dilution.

Recommended Protocol for Immunoblotting using anti-IP3 Receptor Antibodies

NOTE: For protein loading, we recommend 1 x 106 cells/gel lane or 100 μg/gel lane.

• TBST
• 20 mM Tris-HCl, pH 7.6
• 150 mM NaCl
• 0.05% Tween®-20 detergent

1. After transfer, block in 15 ml of TBST with 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody).
2. Dilute primary antibody in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST.
3. Incubate with polyclonal antibody for 1 h or with monoclonal antibody for 2 h.
4. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
5. Dilute secondary antibody also in 5% dry milk (for polyclonal antibody) or 1% BSA or 1-2% gelatin (for monoclonal antibody) in TBST, followed by HRP.
6. Shake for 1 h.
7. Rinse twice rapidly, followed by 1 x 15 min and 2 x 5 min washes with TBST.
8. Mix development solution (1:1) (Amersham ECL kit).
9. In between two pieces of transparency add development solution. Allow to sit for approximately 1 min before exposure to film.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesBourguignon, L.Y.W., et al. 1994. J. Cell Physiol. 159, 29.
Bourguignon, L.Y.W., et al. 1993. J. Biol. Chem. 268, 7290.
Bourguignon, L.Y.W., et al. 1993. Cell Biol. Int. 17, 751.