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428019 Anti-Lamp2 Rat mAb (GL2A7)

428019
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
MRMonoclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
428019-200UL Fiala di plastica 200 ul
Description
OverviewRecognizes the ~60 kDa Lamp2 protein.
Catalogue Number428019
Brand Family Calbiochem®
SynonymsAnti-Lysosome-Associated Membrane Protein 2, Anti-Igp-110
Application Data
Detection of mouse Lamp2 by immunocytochemistry. Sample: Mouse astrocytes fixed in paraformaldehyde. Primary antibody: Anti-Lamp2 Rat mAb (GL2A7) (Cat. No. 428019) (1:100). Detection: fluorescence.
References
ReferencesKannan, K. et al. 1996. Cell Immunol. 171, 10.
Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036.
Chen, J.W., et al. 1985. J. Cell Biol. 101, 85.
Product Information
FormLiquid
FormulationUndiluted ascites.
Positive controlMouse astrocytes
PreservativeNone
Quality LevelMQ100
Applications
Application ReferencesImmunocytochemistry Dharmawardhane, s., et al. 1997. J. Cell. Biol. 138, 1265. Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036. Immunoprecipitation Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036.
Key Applications Immunoblotting (Western Blotting)
Immunocytochemistry
Immunoprecipitation
Application NotesImmunoblotting (1:200)
Immunocytochemistry (1:100, see application references)
Immunoprecipitation (see application references)
Application CommentsVariables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogenmouse liver lysosomal membranes
ImmunogenMouse
CloneGL2A7
HostRat
IsotypeIgG
Species Reactivity
  • Mouse
Antibody TypeMonoclonal Antibody
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Lamp2 Rat mAb (GL2A7) MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Lamp2 Rat mAb (GL2A7) Certificati d'Analisi

TitoloNumero di lotto
428019

Riferimenti bibliografici

Panoramica delle referenze
Kannan, K. et al. 1996. Cell Immunol. 171, 10.
Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036.
Chen, J.W., et al. 1985. J. Cell Biol. 101, 85.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision06-August-2007 RFH
SynonymsAnti-Lysosome-Associated Membrane Protein 2, Anti-Igp-110
ApplicationImmunoblotting (1:200)
Immunocytochemistry (1:100, see application references)
Immunoprecipitation (see application references)
Application Data
Detection of mouse Lamp2 by immunocytochemistry. Sample: Mouse astrocytes fixed in paraformaldehyde. Primary antibody: Anti-Lamp2 Rat mAb (GL2A7) (Cat. No. 428019) (1:100). Detection: fluorescence.
DescriptionRat polyclonal antibody supplied as undiluted ascites. Recognizes the ~60 kDa Lamp2 protein.
BackgroundLysosome-associated membrane protein 2 (Lamp2), also known as lgp2, is a type 1 integral membrane protein that is transported from trans-Golgi network to endosomes and then lysosomes. Upon cell activation, a lamp2 homologue, Lamp1, is transferred to the plasma membrane. Cell surface Lamp1 and 2 have been shown to promote adhesion of human peripheral blood mononuclear cells (PBMC) to vascular endothelium and are possibly involved in the adhesion of PBMC to the site of inflammation.
HostRat
Immunogen speciesMouse
Immunogenmouse liver lysosomal membranes
CloneGL2A7
IsotypeIgG
Speciesmouse
Positive controlMouse astrocytes
FormLiquid
FormulationUndiluted ascites.
PreservativeNone
CommentsVariables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesKannan, K. et al. 1996. Cell Immunol. 171, 10.
Rohrer, J., et al. 1996. J. Cell Biol. 132, 565.
Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036.
Chen, J.W., et al. 1985. J. Cell Biol. 101, 85.
Application referencesImmunocytochemistry Dharmawardhane, s., et al. 1997. J. Cell. Biol. 138, 1265. Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036. Immunoprecipitation Granger, B.L., et al. 1990. J. Biol. Chem. 265, 12036.