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553571 Anti-Ras (31-43) Rabbit pAb

553571
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Species ReactivityHostAntibody Type
B, H, M, R Rb Polyclonal Antibody

Products

Numero di catalogoConfezionamento Qtà/conf
553571-50UG Fiala di plastica 50 μg
Description
OverviewRecognizes all forms of wild type Ras, including the mutant G12V Ras protein.
Catalogue Number553571
Brand Family Calbiochem®
SynonymsAnti-Ras GDP/GTP Binding Protein
Application Data
Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
References
ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
Product Information
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Positive controlRat brain tissue
Preservative≤0.1% sodium azide
Quality LevelMQ100
Applications
Key Applications Immunoblotting (Western Blotting)
Application NotesImmunoblotting (1 µg/ml)
Application CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
Biological Information
Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
HostRabbit
IsotypeIgG
Species Reactivity
  • Bovine
  • Human
  • Mouse
  • Rat
Antibody TypePolyclonal Antibody
Concentration Label Please refer to vial label for lot-specific concentration
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage -20°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Anti-Ras (31-43) Rabbit pAb MSDS

Titolo

Scheda di sicurezza (MSDS) 

Anti-Ras (31-43) Rabbit pAb Certificati d'Analisi

TitoloNumero di lotto
553571

Riferimenti bibliografici

Panoramica delle referenze
Hardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision27-January-2009 RFH
SynonymsAnti-Ras GDP/GTP Binding Protein
ApplicationImmunoblotting (1 µg/ml)
Application Data
Detection of rat Ras by immunoblotting. Sample: Whole tissue extract from rat brain. Primary antibody: Anti-Ras (31-43) Rabbit pAb (Cat. No. 553571) (1 µg/ml). Detection: chemiluminescence.
DescriptionImmunoaffinity rabbit polyclonal antibody. Recognizes all forms of wild type Ras and the mutant G12V Ras protein.
BackgroundRas proteins bind GDP and GTP with high affinity. This region of Ras is important in the binding of Ras to its effector, Raf1. This sequence (residues 31-43) is nearly 100% conserved between the different Ras encoded proteins (H-, K-, and N-Ras) and through phylogeny from slime mold to man.
HostRabbit
Immunogena synthetic peptide [EYDPTIEDSYRKQ(C)] corresponding to amino acids 31-43 in the effector binding Loop (L2) of human H-, K-, and N-Ras, conjugated to KLH
IsotypeIgG
Speciesbovine, human, mouse, rat
Positive controlRat brain tissue
FormLiquid
FormulationIn PBS, 50% glycerol, pH 7.2.
Concentration Label Please refer to vial label for lot-specific concentration
Preservative≤0.1% sodium azide
CommentsBy immmunoblotting, this anti-pan-Ras polyclonal antibody reacts strongly with recombinant Ras fusion protein as well as with recombinant glutathione S-transferase (GST) human Ras and [G12V]GST human Ras. Does not cross-react with members of the Rho or Rab families of Ras-related small G-proteins. No specific reactivity is detected by immunoblotting with rat, mouse or human cell lines or tissue extracts. Variables associated with assay conditions will dictate the proper working dilution.
Storage -20°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-20°C).
Toxicity Standard Handling
ReferencesHardie, G. and Hanks, S. 1995. in The Protein Kinase Facts Book, Protein-Serine Kinases p. 418, Academic Press, San Diego, CA.
Sawada, M., et al. 1989. Nucleic Acid Res. 17, 8867.