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218783 Caspase-1, Human, Recombinant, E. coli

218783
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
218783-3000U Fiala di plastica 3000 u
Description
OverviewRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
Catalogue Number218783
Brand Family Calbiochem®
SynonymsIL-1β Converting Enzyme, ICE
References
ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.
Product Information
Activity≥50,000 units/ml
Unit of DefinitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-<i>p</i>NA (<a href ="/Products/ProductDisplay.asp→catNO=400025">Cat. No. 400025</a>) per min at 30°C, pH 7.4.
FormLiquid
FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
Quality LevelMQ100
Applications
Biological Information
Purity≥90% by SDS-PAGE
Specific Activity≥25,000 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Caspase-1, Human, Recombinant, E. coli MSDS

Titolo

Scheda di sicurezza (MSDS) 

Caspase-1, Human, Recombinant, E. coli Certificati d'Analisi

TitoloNumero di lotto
218783

Riferimenti bibliografici

Panoramica delle referenze
Thornberry, N.A. 1994. Methods Enzymol. 244, 615.
Thornberry, N.A., et al. 1992. Nature 356, 768.

Brochure

Titolo
Caspases and other Apoptosis Related Tools Brochure

Citazioni

Titolo
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.
  • Scheda tecnica

    Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

    Revision23-March-2009 JSW
    SynonymsIL-1β Converting Enzyme, ICE
    DescriptionRecombinant, human caspase-1 fused at the N-terminus to a His•Tag® sequence and expressed in E. coli. Useful for the study of enzyme regulation, cleavage of target substrates, and inhibitor screening. M.W. 10000 and 20000.
    FormLiquid
    FormulationIn 100 mM NaCl, 50 mM HEPES, 10 mM DTT, 1 mM EDTA, 10% glycerol, 0.5% CHAPS, pH 7.4.
    Purity≥90% by SDS-PAGE
    Specific activity≥25,000 units/mg protein
    Activity≥50,000 units/ml
    Unit definitionOne unit is defined as the amount of enzyme that will cleave 1.0 pmol Ac-YVAD-pNA (Cat. No. 400025) per min at 30°C, pH 7.4.
    Storage ≤ -70°C
    Avoid freeze/thaw
    Do Not Freeze Ok to freeze
    Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
    Toxicity Standard Handling
    ReferencesThornberry, N.A. 1994. Methods Enzymol. 244, 615.
    Thornberry, N.A., et al. 1992. Nature 356, 768.
    Citation
  • Sinead M. Miggin, et al. (2007) NF-κB activation by the Toll-IL-1 receptor domain protein MyD88 adapter-like is regulated by caspase-1. Procedings of the National Academy of Science 104, 3372-3377.