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219362 Cathepsin B, Human Liver

219362
Purchase on Sigma-Aldrich

Panoramica

Replacement Information

Tabella delle specifiche principali

Products

Numero di catalogoConfezionamento Qtà/conf
219362-50UG Fiala di plastica 50 μg
Description
OverviewNative cathepsin B from human liver, purified by affinity chromatography and HPLC. The most investigated enzyme of all lysosomal cysteine proteases. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities. Cathepsin B is shown to facilitate tumor invasion by dissolving extracellular barriers.
Catalogue Number219362
Brand Family Calbiochem®
References
ReferencesHirai, K., et al. 1999. Hum. Pathol. 30, 680.
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Strojnik, T., et al. 1999. Clin. Cancer Res. 5, 559.
Maquire, T.M., et al. 1998. Int. J. Biol. Markers 13, 139.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.
Product Information
CAS number9047-22-7
Unit of DefinitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-β-naphthylamide per min at 40°C, using 100 mM Na<sup>+</sup>/K<sup>+</sup> pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
EC number3.4.22.1
FormLiquid
FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
Quality LevelMQ100
Applications
Biological Information
Purity≥95% by SDS-PAGE
SourcePrepared from tissues of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
Specific Activity≥10 units/mg protein
Physicochemical Information
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Dry Ice Only
Toxicity Standard Handling
Storage ≤ -70°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Cathepsin B, Human Liver MSDS

Titolo

Scheda di sicurezza (MSDS) 

Cathepsin B, Human Liver Certificati d'Analisi

TitoloNumero di lotto
219362

Riferimenti bibliografici

Panoramica delle referenze
Hirai, K., et al. 1999. Hum. Pathol. 30, 680.
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Strojnik, T., et al. 1999. Clin. Cancer Res. 5, 559.
Maquire, T.M., et al. 1998. Int. J. Biol. Markers 13, 139.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.

Brochure

Titolo
Cathepsins and Related Products Technical Bulletin
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision18-May-2010 JSW
DescriptionNative cathepsin B from human liver, purified by affinity chromatography and HPLC. The most investigated enzyme of all lysosomal cysteine proteases. Upregulation and secretion of cathepsin B have been shown to occur in many types of tumors and to correlate positively with their invasive and metastatic capabilities.
FormLiquid
FormulationIn 50 mM sodium acetate buffer, 1 mM EDTA, pH 5.0.
SourcePrepared from tissues of individuals that have been shown by certified tests to be negative for HBsAg and for antibodies to HIV and HCV.
CAS number9047-22-7
EC number3.4.22.1
Purity≥95% by SDS-PAGE
Specific activity≥10 units/mg protein
Unit definitionOne unit is defined as the amount of enzyme that will hydrolyze 1.0 µmol Z-RR-β-naphthylamide per min at 40°C, using 100 mM Na+/K+ pH 6.0, with 1.33 mM EDTA and 2 mM DTT as the activation buffer.
Storage ≤ -70°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing initial thaw, aliquot and freeze (-70°C).
Toxicity Standard Handling
Merck USA index14, 1905
ReferencesHirai, K., et al. 1999. Hum. Pathol. 30, 680.
Kostoulas, G., et al. 1999. FEBS Lett. 455, 286.
Strojnik, T., et al. 1999. Clin. Cancer Res. 5, 559.
Maquire, T.M., et al. 1998. Int. J. Biol. Markers 13, 139.
Berquim, I.M., and Sloane, B.F. 1996. Adv. Exp. Med. Biol. 389, 281.