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Supelco

Chromolith® HighResolution RP-18 endcapped (monolithic) HPLC Columns

L × I.D. 100 mm x 4.6 mm HPLC Column

Sinonimo/i:

Chromolith® HighResolution RP-18 endcapped 100-4.6 HPLC column

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$1,160.00

Informazioni su questo articolo

Codice UNSPSC:
41115709
NACRES:
SB.52

$1,160.00

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Product Name

Chromolith® HighResolution RP-18 endcapped, L × I.D. 100 mm x 4.6 mm HPLC column

Materiali

PEEK column

Livello qualitativo

agenzia

suitable for USP L1

Nome Commerciale

Chromolith®

Caratteristiche

endcapped
endcapped

Confezionamento

1 ea of

Grado di funzionalizzazione

15% Carbon loading
18% Carbon loading

Parametri

200 bar pressure
200 bar pressure
45 °C max. temp.
50 °C max. temp.

tecniche

HPLC: suitable
HPLC: suitable
LC/MS: suitable
mass spectrometry (MS): suitable (High)
mass spectrometry (MS): suitable (High)

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Questo articolo
1.520201.520231.52021
matrix active group

C18 bonding phase, octadecylsilane (ODS)

matrix active group

C18 bonding phase, octadecylsilane (ODS)

matrix active group

C18 bonding phase, octadecylsilane (ODS)

matrix active group

C18 bonding phase, octadecylsilane (ODS)

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

separation technique

reversed phase

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

product line

Chromolith®

pore size

1.15 μm macroporosity, 15 nm mesoporosity, 1.5 μm macroporosity

pore size

1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity

pore size

1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity

pore size

1.15 μm macroporosity, 1.5 μm macroporosity, 15 nm mesoporosity

matrix

highly porous, monolithic silica

matrix

highly porous, monolithic silica

matrix

highly porous, monolithic silica

matrix

highly porous, monolithic silica

agency

suitable for USP L1

agency

suitable for ISO/CEN 6304.09, suitable for USP L1

agency

suitable for USP L1

agency

suitable for USP L1

Descrizione generale

Chromolith® columns are made of monolithic silica with a characteristic bimodal pore structure.

Chromolith® HighResolution RP-18 endcapped columns have macropores with 1.15 μm diameter, giving a column efficiency exceeding 140,000 plates/meter. The mesopores are 15 nm (150 Å), the surface modification is octadecylsilane with full end-capping. Chromolith® HighResolution RP-18 endcapped columns fall under the USP L1 Classification. The column efficiency is comparable to a 2.7 to 3 μm particle size fully porous particulate column. Chromolith® HighResolution RP-18 endcapped columns are perfectly suitable for LC-MS applications.
The column dimension of 100 mm length and 4.6 mm I.D. is the best choice for high resolution separations of complex compound mixtures on any HPLC System.

The revolutionary, monolithic silica with its unique combination of macropores and mesopores enables:
—Rapid separations at very low column back-pressure
—High matrix tolerance, allowing time and cost savings with simplified workflows
—Cost-savings due to extended column lifetimes
—Connection of columns, flow gradients and the use of mobile phases with higher viscosity
The recommended Guard-columns are:
Chromolith® HighResolution RP-18 endcapped 5-4.6 guard cartridges (3 pieces) [1.52025] with the Chromolith® Guard Cartridge Holder 5 mm x 4.6 mm [1.52032]
The Chromolith® column coupler can be used to significantly increase the separation efficiency of Chromolith® columns by coupling several columns in series (up to 100,000 plates/per column). [1.51467]

Learn more:
Brochure: Race through your Separations on Any System. Chromolith® Monolithic Silica HPLC Columns

All Supelco HPLC Columns, including monolithic Chromolith® columns, are fully compatible to all HPLC and UHPLC Instruments.
Supelco HPLC Products: The Best Choice for Any LC Instrument

Chromolith® HighResolution RP-18 endcapped columns are also available in 2 mm I.D. column dimension for perfect fit to UHPLC and UHPLC-MS needs.
Flyer: UHPLC-like performance - Rapid and Robust at Low Pressure - Chromolith® HighResolution RP-18 endcapped 2 mm I.D. HPLC Columns

Applicazioni


  • A Novel and Sensitive LC-MS/MS Method for the Quantitation of Ceftiofur in Pharmaceutical Preparations and Milk Samples.: This study, conducted by Levent et al., introduces a highly sensitive LC-MS/MS method for quantifying ceftiofur in pharmaceutical preparations and milk samples. The method utilizes Chromolith® HighResolution RP-18 endcapped columns, showcasing their application in enhancing the resolution and sensitivity of chromatographic analysis in complex matrices (Levent et al., 2021).

Risultati analitici

Appearance: passes test
Pressure: 40 - 70 bar
stability testing: passes test
Performance (anthracene)
Theoretical Plates (N/m) (Anthracene): ≥ 140000
Capacity factor (Anthracene): 3.9 - 4.6
Tailing (USP): 0.90 - 1.30 %
Selectivity
separation factor (anthracene/progesterone): 1.95 - 2.35

Note legali

Chromolith is a registered trademark of Merck KGaA, Darmstadt, Germany

Codice della classe di stoccaggio

11 - Combustible Solids


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Articoli

HPLC-UV determination of 5-HMF (5-(hydroxymethyl)furfural) in beer samples using monolithic silica Chromolith® HighResolution RP-18e HPLC column.

Monolithic silica HPLC columns enable high-throughput analysis at low back pressure without loss of separation efficiency or peak capacity.

Protocolli

Folic acid vs. folate differences and biological activities; implications in fortified foods and supplements for human health.

Contenuto correlato

Small molecules are ions and compounds of molecular weight typically less than 900 daltons. These compounds can be effectively separated and analyzed by HPLC, UHPLC and LC-MS using mainly silica particles or monolithic stationary phases with a broad range of column chemistries (modifications).

Le piccole molecole sono ioni e composti di peso molecolare tipicamente inferiore a 900 dalton. Questi composti possono essere efficacemente separati e analizzati mediante HPLC, UHPLC e LC-MS utilizzando principalmente particelle di silice o fasi stazionarie monolitiche con un'ampia gamma di chimiche delle colonne (modifiche).

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Il team dei nostri ricercatori vanta grande esperienza in tutte le aree della ricerca quali Life Science, scienza dei materiali, sintesi chimica, cromatografia, discipline analitiche, ecc..

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