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322327 Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem

322327
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Replacement Information

Tabella delle specifiche principali

CAS #
92092-36-9

Products

Numero di catalogoConfezionamento Qtà/conf
322327-1MG Fiala di plastica 1 mg
Description
OverviewCRM197 is a nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A, resulting in the complete loss of enzymatic activity. Does not catalyze the ADP ribosylation of EF-2 factor. Immunologically, the CRM197 protein is identical to the wild-type toxin.
Catalogue Number322327
Brand Family Calbiochem®
References
ReferencesKusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
Kaefer, M., et al. 2000. J. Urol. 163, 580.
Gupta, R.K., et al. 1997. Vaccine 15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
Product Information
CAS number92092-36-9
FormSolid
FormulationLyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
ReversibleN
Quality LevelMQ100
Applications
Biological Information
Primary TargetMutant with loss of enzymatic activity to catalyze the ADP ribosylation of EF-2 factor
PurityOne major band by SDS-PAGE
Physicochemical Information
Cell permeableN
Dimensions
Materials Information
Toxicological Information
Safety Information according to GHS
Safety Information
Product Usage Statements
Storage and Shipping Information
Ship Code Ambient Temperature Only
Toxicity Standard Handling
Storage +2°C to +8°C
Avoid freeze/thaw Avoid freeze/thaw
Do not freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
Canadian export regulations Due to the country and/or U.S. state of origin of the animal material used in this product, this product may not be exported to Canada.
Packaging Information
Transport Information
Supplemental Information
Specifications

Documentation

Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem MSDS

Titolo

Scheda di sicurezza (MSDS) 

Diphtheria Toxin, CRM Mutant - CAS 92092-36-9 - Calbiochem Certificati d'Analisi

TitoloNumero di lotto
322327

Riferimenti bibliografici

Panoramica delle referenze
Kusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
Kaefer, M., et al. 2000. J. Urol. 163, 580.
Gupta, R.K., et al. 1997. Vaccine 15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.
Scheda tecnica

Note that this data sheet is not lot-specific and is representative of the current specifications for this product. Please consult the vial label and the certificate of analysis for information on specific lots. Also note that shipping conditions may differ from storage conditions.

Revision07-February-2011 JSW
DescriptionCross-reactive material of diphtheria toxin (CRM197). A nontoxic diphtheria toxin that has a glycine to glutamic acid mutation at amino acid 52 in fragment A that results in complete loss of enzymatic activity. Inhibits smooth muscle cell proliferation in a dose-dependent manner. Immunologically, the CRM197 is identical to the wild-type toxin and is useful as a carrier protein of conjugate vaccines.
FormSolid
FormulationLyophilized from 10 mM sodium phosphate buffer, 5% lactose, pH 7.4.
CAS number92092-36-9
PurityOne major band by SDS-PAGE
SolubilityPlease refer to vial label for lot-specific reconstitution volume.
Storage +2°C to +8°C
Avoid freeze/thaw
Do Not Freeze Ok to freeze
Special InstructionsFollowing reconstitution, aliquot and freeze (-70°C). Stock solutions are stable for up to 1 year at -70°C.
Toxicity Standard Handling
ReferencesKusano, I., et al. 2001. Cell Struct. Funct. 26, 279.
Kaefer, M., et al. 2000. J. Urol. 163, 580.
Gupta, R.K., et al. 1997. Vaccine 15, 1341.
Bixler, G.S., et al. 1989. Adv. Exp. Med. Biol. 251, 175.
Giannini, G., et al. 1984. Nucleic Acids Res. 12, 4063.
Uchida, T., et al. 1973. J. Biol. Chem. 248, 3851.
Pappenheimer, Jr., A.M., et al. 1972. Immunochem. 9, 891.